Abstract

We shall continue determinations of changes in DNA base sequence in forward mutagenesis of lambda phage. Mutagens to be studied include: ultraviolet light, ionizing radiations, benzpyrene, nitrous acid, heat, acridine derivatives, and others. We shall also study the action of mutagens on known base sequences by meaasuring induced reversion to wild type of phage mutants which have been sequenced. We shall interpret the results in terms of the lesions causing the mutations, and the enzymatic mechanisms involved. Because of correlations between mutagenicity and carcinogenicity of various agents, and the recent identification of cellular transforming genes, the results should also be relevant to carcinogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM028297-06
Application #
3275596
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1980-08-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code

  Publications

Sockett, H; Romac, S; Hutchinson, F (1991) DNA sequence changes in mutations induced by ultraviolet light in the gpt gene on the chromosome of Escherichia coli uvr+ and urvA cells. Mol Gen Genet 230:295-301
Sockett, H; Romac, S; Hutchinson, F (1991) Mutagenic specificity of N-methyl-N'-nitro-N-nitrosoguanidine in the gpt gene on a chromosome of Chinese hamster ovary cells and of Escherichia coli cells. Mol Gen Genet 227:252-9
Tindall, K R; Stein, J; Hutchinson, F (1988) Changes in DNA base sequence induced by gamma-ray mutagenesis of lambda phage and prophage. Genetics 118:551-60
Hutchinson, F; Yamamoto, K; Stein, J et al. (1988) Effect of photoreactivation on mutagenesis of lambda phage by ultraviolet light. J Mol Biol 202:593-601
Reed, J; Hutchinson, F (1987) Effect of the direction of DNA replication on mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine in adapted cells of Escherichia coli. Mol Gen Genet 208:446-9
Hutchinson, F (1987) A review of some topics concerning mutagenesis by ultraviolet light. Photochem Photobiol 45:897-903
Wood, R D; Hutchinson, F (1987) Ultraviolet light-induced mutagenesis in the Escherichia coli chromosome. Sequences of mutants in the cI gene of a lambda lysogen. J Mol Biol 193:637-41
Wood, R D; Stein, J (1986) Role of the RecF gene product in UV mutagenesis of lambda phage. Mol Gen Genet 204:82-4
Wood, R D; Hutchinson, F (1986) The role of pyrimidine dimers in mutagenesis by ultraviolet light. Prog Clin Biol Res 209A:191-8
Hutchinson, F; Wood, R D (1986) Mechanisms of mutagenesis of E. coli by ultraviolet light. Basic Life Sci 38:377-83

Showing the most recent 10 out of 12 publications