The ultimate goal of this project is to understand the binding of drugs to nucleic acids in order to provide a foundation for the rational design of new drugs. The new method of fluorescence detected circular dichroism (FDCD) will be used to compare drug binding under various conditions. FDCD measures the difference in fluorescence intensity excited by left and right circularly polarized light. It provides a sensitive probe for conformation around a fluorescent drug. Since fluorescence has high detection sensitivity and specificity, it can be used to study drugs at low concentrations in complex systems. Drug binding in simple and complex systems will be compared. For example, is drug binding in vivo similar to drug binding to free DNA in solution? The effects of drug combinations on binding will also be considered.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM028533-06
Application #
3275797
Study Section
Biophysics and Biophysical Chemistry A Study Section (BBCA)
Project Start
1980-12-01
Project End
1988-11-30
Budget Start
1986-12-01
Budget End
1988-11-30
Support Year
6
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of Rochester
Department
Type
Schools of Arts and Sciences
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627