Abstract

We are attempting to understand the mechanism of gene control in higher organisms by focusing on the expression of the gene that codes for alcohol dehydrogenase in Drosophila melanogaster. Using a variety of biochemical and molecular techniques, we are studying several mutants that lack alcohol dehydrogenase activity. One class of such mutants seems not to synthesize an alcohol dehydrogenase-like protein, even though these mutants have the Adh-gene and make substantial amounts of a mRNA that reacts with an Adh-gene probe. We are currently cloning all the representatives in this class of mutants and hope, by DNA sequencing, to determine what causes this molecular phenotype.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM028791-06
Application #
3276079
Study Section
Genetics Study Section (GEN)
Project Start
1980-07-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Rutgers University
Department
Type
Organized Research Units
DUNS #
038633251
City
New Brunswick
State
NJ
Country
United States
Zip Code

  Publications

Freidman, R; Hotaling, E; Borack, L et al. (1996) Interactions between the regulatory regions of two Adh alleles. Genetica 97:1-14
Shen, N L; Sofer, W H (1991) Introduction of single-stranded ADH genes into Drosophila results in tissue-specific expression. Biochem Biophys Res Commun 174:1300-5
Shen, N L; Hotaling, E C; Subrahmanyam, G et al. (1991) Analysis of sequences regulating larval expression of the Adh gene of Drosophila melanogaster. Genetics 129:763-71
Rothberg, I; Hotaling, E; Sofer, W (1991) A Drosophila Adh gene can be activated in trans by an enhancer. Nucleic Acids Res 19:5713-7
Shen, N L; Subrahmanyam, G; Clark, W et al. (1989) Analysis of Adh gene regulation in Drosophila: studies using somatic transformation. Dev Genet 10:210-9
Sofer, W; Martin, P (1987) Analysis of densitometric data obtained from electrophoretic analysis. Comput Appl Biosci 3:129
Fargnoli, J; Sofer, W (1987) In situ detection of enzymes in yeast. Anal Biochem 162:384-8
Sofer, W; Martin, P F (1987) Analysis of alcohol dehydrogenase gene expression in Drosophila. Annu Rev Genet 21:203-25
Fargnoli, J; Hyde, J; Sofer, W (1987) Chemical selection for beta-galactosidase activity in Drosophila melanogaster. Biochem Genet 25:327-33
Place, A R; Benyajati, C; Sofer, W (1987) Molecular consequences of two formaldehyde-induced mutations in the alcohol dehydrogenase gene of Drosophila melanogaster. Biochem Genet 25:621-38

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