Abstract

The molecular organization of the photosynthetic membrane and its components will be investigated by high resolution electron microscope techniques. Three systems will be used for study: a bacterial photosynthetic membrane, photosynthetic membranes from higher plants in which ordered lattices of membrane subunits have been induced, and a reconstituted system containing a chlorophyll-protein complex in an artificial lipid bilayer. Each of these systems has shown a high degree of two-dimensional order, and this characteristic will be exploited in analyzing their detailed substructure. Electron and optical diffraction techniques, combined with computer analysis of electron micrographs, will be used to form high resolution synthesized images of the membrane systems. It is expected that these high resolution maps will provide insights into the nature of the photosynthetic process and the structure of biological membranes in general.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM028799-05
Application #
3276103
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1983-03-01
Project End
1986-02-28
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Brown University
Department
Type
Schools of Medicine
DUNS #
001785542
City
Providence
State
RI
Country
United States
Zip Code

  Publications

Hinshaw, J E; Miller, K R (1993) Mapping the lateral distribution of photosystem II and the cytochrome b6/f complex by direct immune labeling of the thylakoid membrane. J Struct Biol 111:1-8
Hinshaw, J E; Miller, K R (1989) Localization of light-harvesting complex II to the occluded surfaces of photosynthetic membranes. J Cell Biol 109:1725-31
Bassi, R; Ghiretti Magaldi, A; Tognon, G et al. (1989) Two-dimensional crystals of the photosystem II reaction center complex from higher plants. Eur J Cell Biol 50:84-93
Miller, K R; Jacob, J S; Burger-Wiersma, T et al. (1988) Supramolecular structure of the thylakoid membrane of Prochlorothrix hollandica: a chlorophyll b-containing prokaryote. J Cell Sci 91 ( Pt 4):577-86
Jacob, J S; Miller, K R (1986) The effects of galactolipid depletion on the structure of a photosynthetic membrane. J Cell Biol 103:1337-47
Spelman, L H; Thompson, N L; Fausto, N et al. (1986) A structural analysis of gap and tight junctions in the rat liver during a dietary treatment that induces oval cell proliferation. Am J Pathol 125:379-92
Miller, K R; Jacob, J S; Smith, U et al. (1986) Heliobacterium chlorum: cell organization and structure. Arch Microbiol 146:111-4
Miller, K R; Jacob, J S (1985) Two-dimensional crystals of a membrane protein: arrangement of subunits within the crystal sheet. Eur J Cell Biol 36:247-55
Miller, K R (1985) The photosynthetic membrane: prokaryotic and eukaryotic cells. Endeavour 9:175-82
Miller, K R; Jacob, J S (1985) The Rhodopseudomonas viridis photosynthetic membrane: arrangement in situ. Arch Microbiol 142:333-9

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