The long-term ojective of these studies is to understand how the synthesis of specific temporally expressed proteins is regulated in the mammalian testis. Emphasis will be placed upon establishing the function of a unique testicular a-tubulin and defining the cis-acting regulatory sequences in the genomic DNA of this tubulin and several other haploid gene products including the two protamines. Additional studies will explain how coordinate expression of genes in pre-meiotic, meiotic and post-meiotic testicular cells is controlled. Specific projects to be undertaken include: 1) determining the localization in testicular cells by immunofluorescence of the unique post-meiotically synthesized mouse a-tubulin. 2) determining whether a common DNA enhancer sequence regulates the synthesis of the protamine, a-tubulin, and other coordinately expressed haploid genes. 3) identifying specific cDNA and genomic clones for pre-meiotic and meiotic testicular cell-types to allow a direct comparison of genomic regulatory signals to be made between different classes of temporally regulated genes. 4) establishing the relationship between DNA methylation and the gene transcription and nuclear geome condensation with our growing collection of cell-type specific probes. These studies on the molecular basis for gene regulation in the testis will enhance our knowledge of basic control mechanisms during spermatagenesis, provide necessary probes to understand molecular causes of infertility, and provide superior approaches to fertility control.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
Project #
Application #
Study Section
Reproductive Biology Study Section (REB)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Tufts University
Schools of Arts and Sciences
United States
Zip Code
Kwon, Y K; Hecht, N B (1993) Binding of a phosphoprotein to the 3' untranslated region of the mouse protamine 2 mRNA temporally represses its translation. Mol Cell Biol 13:6547-57
Alcivar, A A; Hake, L E; Hecht, N B (1992) DNA polymerase-beta and poly(ADP)ribose polymerase mRNAs are differentially expressed during the development of male germinal cells. Biol Reprod 46:201-7
Hermo, L; Oko, R; Hecht, N B (1991) Differential post-translational modifications of microtubules in cells of the seminiferous epithelium of the rat: a light and electron microscope immunocytochemical study. Anat Rec 229:31-50
Yelick, P C; Kozak, C; Kwon, Y K et al. (1991) The mouse transition protein 1 gene contains a B1 repetitive element and is located on chromosome 1. Genomics 11:687-94
Oko, R; Hermo, L; Hecht, N B (1991) Distribution of actin isoforms within cells of the seminiferous epithelium of the rat testis: evidence for a muscle form of actin in spermatids. Anat Rec 231:63-81
Johnson, P A; Bunick, D; Hecht, N B (1991) Protein binding regions in the mouse and rat protamine-2 genes. Biol Reprod 44:127-34
Kwon, Y K; Hecht, N B (1991) Cytoplasmic protein binding to highly conserved sequences in the 3' untranslated region of mouse protamine 2 mRNA, a translationally regulated transcript of male germ cells. Proc Natl Acad Sci U S A 88:3584-8
Alcivar, A A; Trasler, J M; Hake, L E et al. (1991) DNA methylation and expression of the genes coding for lactate dehydrogenases A and C during rodent spermatogenesis. Biol Reprod 44:527-35
Morales, C R; Kwon, Y K; Hecht, N B (1991) Cytoplasmic localization during storage and translation of the mRNAs of transition protein 1 and protamine 1, two translationally regulated transcripts of the mammalian testis. J Cell Sci 100 ( Pt 1):119-31
Hecht, N B (1990) Regulation of 'haploid expressed genes' in male germ cells. J Reprod Fertil 88:679-93

Showing the most recent 10 out of 36 publications