Abstract

The movement of cellular organelles, e. g. chromosomes, vesicles, etc. is of fundamental importance to biology and medicine. Kinesin is one of a group of mechanochemical proteins known to move organelles along microtubules. Until recently only a single archetypal kinesin protein was known. The description of other kinesin-like proteins in A. nidulans, S. cerevisiae and D. melanogaster during the past year has established the kinesins as a family proteins with type-specific functions. The kinesin-like bimC protein of A. nidulans appears to be of special interest because it is required for nuclear division. This grant proposes to continue the structural and functional characterization of bimC with special emphasis on understanding how it works during mitosis. klpA, a second kinesin-like protein of A. nidulans will also be characterized. Molecular genetics will be used to determine the relationship between structure and function of these proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM029228-14
Application #
2175450
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1981-06-01
Project End
1996-06-30
Budget Start
1994-07-01
Budget End
1996-06-30
Support Year
14
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of Medicine & Dentistry of NJ
Department
Pharmacology
Type
Schools of Medicine
DUNS #
622146454
City
Piscataway
State
NJ
Country
United States
Zip Code
08854

  Publications

Xiang, X; Osmani, A H; Osmani, S A et al. (1995) Analysis of nuclear migration in Aspergillus nidulans. Cold Spring Harb Symp Quant Biol 60:813-9
Beckwith, S M; Roghi, C H; Morris, N R (1995) The genetics of nuclear migration in fungi. Genet Eng (N Y) 17:165-80
Chiu, Y H; Morris, N R (1995) Extragenic suppressors of nudC3, a mutation that blocks nuclear migration in Aspergillus nidulans. Genetics 141:453-64
Willins, D A; Xiang, X; Morris, N R (1995) An alpha tubulin mutation suppresses nuclear migration mutations in Aspergillus nidulans. Genetics 141:1287-98
Kirk, K E; Morris, N R (1993) Either alpha-tubulin isogene product is sufficient for microtubule function during all stages of growth and differentiation in Aspergillus nidulans. Mol Cell Biol 13:4465-76
Morris, N R; Enos, A P (1992) Mitotic gold in a mold: Aspergillus genetics and the biology of mitosis. Trends Genet 8:32-7
Kirk, K E; Morris, N R (1991) The tubB alpha-tubulin gene is essential for sexual development in Aspergillus nidulans. Genes Dev 5:2014-23
Enos, A P; Morris, N R (1990) Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans. Cell 60:1019-27
May, G S; Waring, R B; Morris, N R (1990) Increasing tubC beta-tubulin synthesis by placing it under the control of a benA beta-tubulin upstream sequence causes a reduction in benA beta-tubulin level but has no effect on microtubule function. Cell Motil Cytoskeleton 16:214-20
Osmani, S A; Engle, D B; Doonan, J H et al. (1988) Spindle formation and chromatin condensation in cells blocked at interphase by mutation of a negative cell cycle control gene. Cell 52:241-51

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