Abstract

Using appropriate genetic procedures for the production of isogenic strains of E. coli and appropriate media for positive and negative controls, we have successfully analyzed the selective effects of four alleles determining naturally occurring allozymes of the enzyme 6-phosphogluconate dehydrogenase. The alleles are selectively neutral in the K-12 genetic background. We now propose to carry out similar studies of loci associated with a number of other allozyme polymorphisms, including alcohol dehydrogenase, glutamic oxaloacetate transferase, glucose-6-phosphate dehydrogenase, phosphoglucose isomerase, glyceraldehyde-3-phosphate dehydrogenase, peptidase, mannose phosphate isomerase, isocitrate dehydrogenase, and phosphoglucomutase. Possible multiple locus effects on fitness will also be examined, as will certain biochemical characteristics of allozymes found to be associated with selection. These studies bear on the hypothesis of selective neutrality as it pertains to allozymes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM030201-05
Application #
3277831
Study Section
Genetics Study Section (GEN)
Project Start
1981-09-01
Project End
1986-09-04
Budget Start
1985-09-01
Budget End
1986-09-04
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Guttman, D S; Dykhuizen, D E (1994) Detecting selective sweeps in naturally occurring Escherichia coli. Genetics 138:993-1003
Dykhuizen, D E (1993) Chemostats used for studying natural selection and adaptive evolution. Methods Enzymol 224:613-31
Sawyer, S A; Hartl, D L (1992) Population genetics of polymorphism and divergence. Genetics 132:1161-76
Dykhuizen, D E; Green, L (1991) Recombination in Escherichia coli and the definition of biological species. J Bacteriol 173:7257-68
DuBose, R F; Hartl, D L (1990) Rapid purification of PCR products for DNA sequencing using Sepharose CL-6B spin columns. Biotechniques 8:271-4
DuBose, R F; Hartl, D L (1990) The molecular evolution of bacterial alkaline phosphatase: correlating variation among enteric bacteria to experimental manipulations of the protein. Mol Biol Evol 7:547-77
Hartl, D L (1989) Evolving theories of enzyme evolution. Genetics 122:1-6
Hartl, D L (1989) The physiology of weak selection. Genome 31:183-9
DuBose, R F; Hartl, D L (1989) An experimental approach to testing modular evolution: directed replacement of alpha-helices in a bacterial protein. Proc Natl Acad Sci U S A 86:9966-70
Lawrence, J G; Dykhuizen, D E; DuBose, R F et al. (1989) Phylogenetic analysis using insertion sequence fingerprinting in Escherichia coli. Mol Biol Evol 6:1-14

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