Abstract

The long-term objective of this work is to develop improved methods for identifying and characterizing large molecules, and to apply these methods to various biological problems. The methods include time-of-flight techniques for secondary ion mass spectrometry (SIMS), and the combination of liquid chromatography with time-of-flight mass spectrometry. Specifically it is proposed: 1. To make improvements in instrumentation and technique, including modifications to an existing time-of-flight spectrometer, construction of a new time-of-flight spectrometer of unusual design, and improvements to an existing liquid chromatograph. 2. To carry out studies on the basic physics of the secondary ion emission process, so as to obtain a better understanding of the process and to facilitate its application. 3. To apply time-of-flight mass spectrometry and its combination with liquid chromatography (LC/MS) to various samples of biological interest, in particular to fully protected nucleotides and natural mixtures of neuropeptides.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM030605-05
Application #
3278402
Study Section
(BET)
Project Start
1982-06-01
Project End
1988-05-31
Budget Start
1986-06-01
Budget End
1987-05-31
Support Year
5
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Manitoba
Department
Type
DUNS #
207584707
City
Winnipeg
State
MB
Country
Canada
Zip Code
R3 2N2

  Publications

Lei, Q P; Cui, X; Kurtz Jr, D M et al. (1998) Electrospray mass spectrometry studies of non-heme iron-containing proteins. Anal Chem 70:1838-46
Rose, K; Zeng, W; Regamey, P O et al. (1996) Natural peptides as building blocks for the synthesis of large protein-like molecules with hydrazone and oxime linkages. Bioconjug Chem 7:552-6
Donald, L J; Chernushevich, I V; Zhou, J et al. (1996) Preparation and properties of pure, full-length IclR protein of Escherichia coli. Use of time-of-flight mass spectrometry to investigate the problems encountered. Protein Sci 5:1613-24
Sevinc, M S; Ens, W; Loewen, P C (1995) The cysteines of catalase HPII of Escherichia coli, including Cys438 which is blocked, do not have a catalytic role. Eur J Biochem 230:127-32
Verentchikov, A N; Ens, W; Standing, K G (1994) Reflecting time-of-flight mass spectrometer with an electrospray ion source and orthogonal extraction. Anal Chem 66:126-33
Tang, X J; Brewer, C F; Saha, S et al. (1994) Investigation of protein-protein noncovalent interactions in soybean agglutinin by electrospray ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 8:750-4
Tocchio, R M; Williams, P T; Mayer, F J et al. (1993) Laser debonding of ceramic orthodontic brackets. Am J Orthod Dentofacial Orthop 103:155-62
Ens, W; Main, D E; Standing, K G et al. (1988) Comparison of relative quasi-molecular ion yields for 8-keV ion and 252Cf fission fragment bombardment. Anal Chem 60:1494-8
Main, D E; Ens, W; Beavis, R et al. (1987) Redox reactions occurring during secondary ion mass spectrometry of some heteroanthracycline antitumor antibiotic precursors. Biomed Environ Mass Spectrom 14:91-6
Lafortune, F; Beavis, R; Tang, X et al. (1987) Narrowing the gap between keV and fission fragment secondary ion yields with nitrocellulose. Rapid Commun Mass Spectrom 1:114-6

Showing the most recent 10 out of 11 publications