Abstract

In the proposed program we plan to study novel human glycosyltransferases. Much emphasis will be made on the identification and characterization of enzymes present in ovarian epithelial linings and fluids. Synthesis of various oligosaccharides which can be effectively employed as acceptor-substrates for certain glycosyltransferases have already been accomplished in our laboratory. A number of these synthetic substrates have proven to be very useful and valuable acceptors for glycosyltransferases from sources other than human. Our main strategy for the characterization of the product resulting from the reaction of a glycosyltransferase and its acceptor involves the synthesis of the expected saccharide which can be used as a reference compound. The structure of the saccharide isolated from enzymatic product will be established by n.m.r., mass spectroscopy, and methylation studies. We plan to employ glycosidases for the structural studies of the new product. Adequate use of newly acquired HPLC instrumentation will be made to facilitate these investigations. The proposed studies are directed toward the characterization of the spectrum of glycosyltransfeerases which are responsible for elongation of carbohydrate units O-glycosidically linked to Serine or Threonine. We are concentrating on the study of glycosyltransferases which have not been identified but whose existence is inferred from structural studies and the """"""""one enzyme-one linkage"""""""" concept. Our studies will further elucidate the biosynthetic pathway of glycoconjugates. It is anticipated that glycosyltransferases of defined specificity, as proposed in this program in conjunction with glycosidases, may be of general value in further understanding the roles of specific oligosaccharide structures in the complex mileu of cell-surface glycoconjugates.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM030727-02
Application #
3278560
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1983-12-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Roswell Park Cancer Institute Corp
Department
Type
DUNS #
City
Buffalo
State
NY
Country
United States
Zip Code
14263