This project aims to characterize direct interactions between RNA molecules and bilayer membranes, and to manipulate these interactions for experimental purposes. As a consequence of these studies, such interactions will be sought in cells. In addition, this this project proposes to find and characterize new classes of RNA enzymes that use enzymatic cofactors as and intrinsic part of their reaction mechanisms.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM030881-23
Application #
6909998
Study Section
Physiological Chemistry Study Section (PC)
Program Officer
Rhoades, Marcus M
Project Start
1982-07-01
Project End
2007-06-30
Budget Start
2005-07-01
Budget End
2007-06-30
Support Year
23
Fiscal Year
2005
Total Cost
$327,711
Indirect Cost
Name
University of Colorado at Boulder
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
007431505
City
Boulder
State
CO
Country
United States
Zip Code
80309
Janas, Teresa; Janas, Tadeusz; Yarus, Michael (2012) Human tRNA(Sec) associates with HeLa membranes, cell lipid liposomes, and synthetic lipid bilayers. RNA 18:2260-8
Smit, Sandra; Yarus, Michael; Knight, Rob (2006) Natural selection is not required to explain universal compositional patterns in rRNA secondary structure categories. RNA 12:1-14
Janas, Tadeusz; Janas, Teresa; Yarus, Michael (2006) Specific RNA binding to ordered phospholipid bilayers. Nucleic Acids Res 34:2128-36
Knight, Rob; De Sterck, Hans; Markel, Rob et al. (2005) Abundance of correctly folded RNA motifs in sequence space, calculated on computational grids. Nucleic Acids Res 33:5924-35
Janas, Teresa; Janas, Tadeusz; Yarus, Michael (2004) A membrane transporter for tryptophan composed of RNA. RNA 10:1541-9
Janas, Tadeusz; Yarus, Michael (2003) Visualization of membrane RNAs. RNA 9:1353-61
Jadhav, Vasant R; Yarus, Michael (2002) Coenzymes as coribozymes. Biochimie 84:877-88
Illangasekare, Mali; Yarus, Michael (2002) Phenylalanine-binding RNAs and genetic code evolution. J Mol Evol 54:298-311
Jadhav, Vasant R; Yarus, Michael (2002) Acyl-CoAs from coenzyme ribozymes. Biochemistry 41:723-9
Kumar, R K; Yarus, M (2001) RNA-catalyzed amino acid activation. Biochemistry 40:6998-7004

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