Regulatory Proteins in E coli and Eukaryotic Cells

Riggs, Arthur D.

Abstract

We will continue studies on: 1. The biological function of mammalian DNA methylation, with the purpose of proving the involvement of DNA methylation in gene regulation, differentiation, development, and X chromosome inactivation. 2. The molecular mechanism of mammalian X chromosome inactivation. 3. Mammalian X chromosome isolation and characterization. 4. The molecular cloning and sequence characterization of random DNA fragments from the X chromosome. 5. The molecular cloning of sequence characterization of X chromosome specific genes, with emphasis on the gene for phosphoglycerate kinase. 6. Mammalian DNA methylases.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Research Project (R01)
• Project #: 5R01GM031263-05
• Application #: 3279203
• Study Section: Mammalian Genetics Study Section (MGN)
• Project Start: 1981-08-01
• Project End: 1986-11-30
• Budget Start: 1984-12-01
• Budget End: 1985-11-30
• Support Year: 5
• Fiscal Year: 1985

Institution

• Name: City of Hope/Beckman Research Institute
• City: Duarte
• State: CA
• Country: United States
• Zip Code: 91010

Publications

Mas, M T; Resplandor, Z E; Riggs, A D (1987) Site-directed mutagenesis of glutamate-190 in the hinge region of yeast 3-phosphoglycerate kinase: implications for the mechanism of domain movement. Biochemistry 26:5369-77

Vogelstein, B; Fearon, E R; Hamilton, S R et al. (1987) Clonal analysis using recombinant DNA probes from the X-chromosome. Cancer Res 47:4806-13

McCarrey, J R; Riggs, A D (1986) Determinator-inhibitor pairs as a mechanism for threshold setting in development: a possible function for pseudogenes. Proc Natl Acad Sci U S A 83:679-83

Mas, M T; Chen, C Y; Hitzeman, R A et al. (1986) Active human-yeast chimeric phosphoglycerate kinases engineered by domain interchange. Science 233:788-90

Mori, N; Singer-Sam, J; Riggs, A D (1986) Evolutionary conservation of the substrate-binding cleft of phosphoglycerate kinases. FEBS Lett 204:313-7

Mori, N; Singer-Sam, J; Lee, C Y et al. (1986) The nucleotide sequence of a cDNA clone containing the entire coding region for mouse X-chromosome-linked phosphoglycerate kinase. Gene 45:275-80