Cell motility is essential for many biological processes. During development, cells move in a organized manner to give rise to specific organs, and disruption of this process can lead to serious abnormalities. Directed cell movements are also associated with angiogenesis, neuronal re-arrangements, wound healing, and the movement of macrophages to sites of infection. One of the most lethal manifestations of uncontrolled cell motility is metastasis. In muscle, myosis is the protein which converts the chemical energy of ATP into the mechanical energy of movement. Myosin is also present in nonmuscle cells and is believed to be responsible for most types of cell locomotion. In order to understand how cell motility is regulated, therefore, it is essential to study regulation of the myosin molecule. Dictyostelium is an excellent system since cells can be grown easily, are amenable to genetic analysis, and exhibit chemotaxis. A major goal is to understand how myosin assembly and ATPase activity are controlled by the cell. Since filament structure can directly regulate ATPase activity, it is also important to study the relationship between these two processes. The main techniques for studying assembly will be sedimentation in an airfuge and turbidity changes in a stopped-flow spectrophotometer. With the later instrument it should be possible to identify subtle effects which influence the rate of assembly or disassembly. In addition to the effect of various solution conditions on assembly, a search will be made for possible myosin- associated proteins which regulate the assembly or stability of myosin thick filaments. A known regulator of assembly is heavy chain phosphorylation, and detailed studies of phosphorylation effects will be carried out using partially purified kinases. Additionally, an attempt will be made to purify the kinase which phosphorylates a specific in vivo site. To identify domains essential for assembly, a variety of well-defined proteolytic fragments of the tail will be studied for their effect on assembly and ATPase activity. Since filament stability and shape are very sensitive to solution conditions and modifications of the myosin molecule, attempts will be made to stabilize the filaments in a known conformation by use of chemical cross-linkers. This should provide important new information on the relationship between filament structure and ATPase activity.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM031907-11
Application #
2176359
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1983-04-01
Project End
1995-09-30
Budget Start
1993-04-01
Budget End
1995-09-30
Support Year
11
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Rosalind Franklin University
Department
Physiology
Type
Schools of Medicine
DUNS #
069501252
City
North Chicago
State
IL
Country
United States
Zip Code
60064
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Aguado-Velasco, C; Kuczmarski, E R (1993) Contraction of reconstituted Dictyostelium cytoskeletons: an apparent role for higher order associations among myosin filaments. Cell Motil Cytoskeleton 26:103-14
Kuczmarski, E R; Palivos, L; Aguado, C et al. (1991) Stopped-flow measurement of cytoskeletal contraction: Dictyostelium myosin II is specifically required for contraction of amoeba cytoskeletons. J Cell Biol 114:1191-9
Kuczmarski, E R; Routsolias, L; Parysek, L M (1988) Proteolytic fragmentation of Dictyostelium myosin and localization of the in vivo heavy chain phosphorylation site. Cell Motil Cytoskeleton 10:471-81
Watanabe, T K; Kuczmarski, E R; Reddy, J K (1987) Myosin from pancreatic acinar carcinoma cells. Isolation, characterization and demonstration of heavy- and light-chain phosphorylation. Biochem J 247:513-8
Kuczmarski, C A; Orlina, A R; Delahanty, L K et al. (1987) Instability of red cell shape associated with the absence of membrane glycophorin C. Vox Sang 52:36-42
Kuczmarski, E R; Tafuri, S R; Parysek, L M (1987) Effect of heavy chain phosphorylation on the polymerization and structure of Dictyostelium myosin filaments. J Cell Biol 105:2989-97
Kuczmarski, E R; Pagone, J (1986) Myosin specific phosphatases isolated from Dictyostelium discoideum. J Muscle Res Cell Motil 7:510-6
Kuczmarski, E R (1986) Partial purification of two myosin heavy chain kinases from Dictyostelium discoideum. J Muscle Res Cell Motil 7:501-9