Abstract

The following three lines of experimentation are proposed: (1) The definition of the molecular mechanisms of the regulation of transcription of the white locus of Drosophila melanogaster. These studies consist of a detailed physical analysis of white locus elements having apparent regulatory function as assessed by previous molecular and genetic studies. The proposed studies are directed at defining the physical relationship of these elements to the white locus transcription unit and at determining the effects of previously characterized white mutant alleles on the transcription of the locus. The transcription of the locus in wild type and mutant alleles at the locus will be analyzed and the function of white locus genetic elements will be investigated by in vitro mutagenesis and reintroduction of the locus by P element-mediated germline genetic transformation. (2) The test of a novel strategy for germline genetic transformation in Drosophila. The proposed method would allow the introduction of a Drosophila DNA segment at its locus of origin and would have several advantages over P element-mediated transformation. Several experiments are proposed to test and characterize this strategy. (3) The genetic and biochemical analysis of the zeste and suppressor-of-wa loci in Drosophila. By hypothesis, each of these loci is a developmental regulatory locus (one whose function is to regulate the developmental expression of other loci). Both of these loci are readily amenable to genetic manipulation and, in each case, at least one transcription unit presumed to be regulated by these loci is available in cloned form. Studies are proposed to test the hypothesis that these loci are regulatory and to define the nature and function of the products of the two loci.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM032003-03
Application #
3280518
Study Section
Genetics Study Section (GEN)
Project Start
1983-04-01
Project End
1986-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Type
Schools of Arts and Sciences
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Zachar, Z; Chou, T B; Kramer, J et al. (1994) Analysis of autoregulation at the level of pre-mRNA splicing of the suppressor-of-white-apricot gene in Drosophila. Genetics 137:139-50
Zachar, Z; Kramer, J; Bingham, P M (1994) Looking at mRNA splicing and transport in situ. Methods Cell Biol 44:599-611
Spikes, D A; Kramer, J; Bingham, P M et al. (1994) SWAP pre-mRNA splicing regulators are a novel, ancient protein family sharing a highly conserved sequence motif with the prp21 family of constitutive splicing proteins. Nucleic Acids Res 22:4510-9
Zachar, Z; Kramer, J; Mims, I P et al. (1993) Evidence for channeled diffusion of pre-mRNAs during nuclear RNA transport in metazoans. J Cell Biol 121:729-42
Spikes, D; Bingham, P M (1992) Analysis of spliceosome assembly and the structure of a regulated intron in Drosophila in vitro splicing extracts. Nucleic Acids Res 20:5719-27
Zachar, Z; Bingham, P M (1989) Suppressible insertion-induced mutations in Drosophila. Prog Nucleic Acid Res Mol Biol 36:87-98
Bingham, P M; Chou, T B; Mims, I et al. (1988) On/off regulation of gene expression at the level of splicing. Trends Genet 4:134-8
Zachar, Z; Chou, T B; Bingham, P M (1987) Evidence that a regulatory gene autoregulates splicing of its transcript. EMBO J 6:4105-11
Zachar, Z; Garza, D; Chou, T B et al. (1987) Molecular cloning and genetic analysis of the suppressor-of-white-apricot locus from Drosophila melanogaster. Mol Cell Biol 7:2498-505
Bingham, P M; Chapman, C H (1986) Evidence that white-blood is a novel type of temperature-sensitive mutation resulting from temperature-dependent effects of a transposon insertion on formation of white transcripts. EMBO J 5:3343-51

Showing the most recent 10 out of 13 publications