Abstract

During muscle development, changes in gene transcription occur during specific phases of the differentiative process. In the proposed experiments a labeled muscle-specific complementary DNA (cDNA) will be used as a hybridization probe to obtain quantitative information regarding the regulation of expression of muscle-specific genes during myogenesis in vivo. Analysis of the total poly A+RNA population of myoblasts by cDNA hybridization indicates that the bulk of this class of RNA represents transcriptions from """"""""housekeeping genes"""""""" which are expressed in all cells. A small, but measurable fraction of the cDNA to myoblast poly A+RNA is complementary to RNA transcripts which are probably present only in developing muscle. This muscle-specific cDNA will be isolated by preparative hybridization techniques designed to eliminate cDNA complementary to RNA from a non-myogenic tissue (embryonic brain). Extensive tests will indicate whether the cDNA which fails to hybridize to brain RNA is indeed """"""""muscle-specific"""""""". The isolated muscle-specific cDNA will be used to estimate the diversity of the muscle-specific genes as well as determining the intracellular localization and numbers of copies per myoblast cell of the transcripts from muscle-specific genes. The results of these experiments will provide important information regarding the role and mechanisms of the expression of phenotype-specific gene sets during molecular and embryonic differentiation of myogenic cells are well as eucaryotic cells in general.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM032018-04
Application #
3280559
Study Section
Genetics Study Section (GEN)
Project Start
1982-09-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1987-06-30
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Williams, B A; Ordahl, C P (1994) Pax-3 expression in segmental mesoderm marks early stages in myogenic cell specification. Development 120:785-96
Stewart, A F; Larkin, S B; Farrance, I K et al. (1994) Muscle-enriched TEF-1 isoforms bind M-CAT elements from muscle-specific promoters and differentially activate transcription. J Biol Chem 269:3147-50
Antin, P B; Karp, G C; Ordahl, C P (1991) Transgene expression in the QM myogenic cell line. Dev Biol 143:122-9
Iannello, R C; Mar, J H; Ordahl, C P (1991) Characterization of a promoter element required for transcription in myocardial cells. J Biol Chem 266:3309-16
Antin, P B; Ordahl, C P (1991) Isolation and characterization of an avian myogenic cell line. Dev Biol 143:111-21
Mar, J H; Ordahl, C P (1990) M-CAT binding factor, a novel trans-acting factor governing muscle-specific transcription. Mol Cell Biol 10:4271-83
Nikovits Jr, W; Mar, J H; Ordahl, C P (1990) Muscle-specific activity of the skeletal troponin I promoter requires interaction between upstream regulatory sequences and elements contained within the first transcribed exon. Mol Cell Biol 10:3468-82
Antin, P B; Mar, J H; Ordahl, C P (1988) Single cell analysis of transfected gene expression in primary heart cultures containing multiple cell types. Biotechniques 6:640-2, 645-9
Mar, J H; Antin, P B; Cooper, T A et al. (1988) Analysis of the upstream regions governing expression of the chicken cardiac troponin T gene in embryonic cardiac and skeletal muscle cells. J Cell Biol 107:573-85
Nikovits Jr, W; Kuncio, G; Ordahl, C P (1986) The chicken fast skeletal troponin I gene: exon organization and sequence. Nucleic Acids Res 14:3377-90

Showing the most recent 10 out of 13 publications