I propose to develop a computer model of bacterial growth, based on our current understanding of the control of synthesis of ribosomes, RNA polymerase, chromosome replication and cell division that are being studied in my laboratory. I propose to study the implications of this model and to continually refine it as new experimental data become available. The model would simulate physiological experiments like nutritional shifts, changes in replication velocity or inhibition of macromolecule synthesis rates by specific drugs, or the behavior of mutants with a defect, e.g. the control of DNA replication or ribosome synthesis. Such mutants have been isolated and are being studied in our laboratory. The model is expected to improve our understanding of bacterial physiology and to serve as an aid for data evaluation and guide for experimentation.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM032074-01A1
Application #
3280639
Study Section
(SSS)
Project Start
1984-12-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Texas-Dallas
Department
Type
Schools of Arts and Sciences
DUNS #
City
Richardson
State
TX
Country
United States
Zip Code
75080
Baracchini, E; Bremer, H (1987) Determination of synthesis rate and lifetime of bacterial mRNAs. Anal Biochem 167:245-60
Bremer, H; Lin-Chao, S (1986) Analysis of the physiological control of replication of ColE1-type plasmids. J Theor Biol 123:453-70