Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
3R01GM032508-08S1
Application #
2176614
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1983-08-01
Project End
1994-08-31
Budget Start
1992-09-01
Budget End
1993-08-31
Support Year
8
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Carnegie-Mellon University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
052184116
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Bucci, M; Moyer, T W; Brown, C M et al. (1994) The receptor-recycling and lysosome biogenesis mutant TfT1.11 belongs to a new complementation group, End6. Somat Cell Mol Genet 20:47-54
Brockman, S A; Murphy, R F (1994) Isolation and analysis of somatic cell mutants with defects in endocytic traffic. Methods Cell Biol 42 Pt B:131-48
Murphy, R F; Schmid, J; Fuchs, R (1993) Endosome maturation: insights from somatic cell genetics and cell-free analysis. Biochem Soc Trans 21 ( Pt 3):716-20
Wilson, R B; Mastick, C C; Murphy, R F (1993) A Chinese hamster ovary cell line with a temperature-conditional defect in receptor recycling is pleiotropically defective in lysosome biogenesis. J Biol Chem 268:25357-63
Cain, C C; Wilson, R B; Murphy, R F (1991) Isolation by fluorescence-activated cell sorting of Chinese hamster ovary cell lines with pleiotropic, temperature-conditional defects in receptor recycling. J Biol Chem 266:11746-52
Sipe, D M; Jesurum, A; Murphy, R F (1991) Absence of Na+,K(+)-ATPase regulation of endosomal acidification in K562 erythroleukemia cells. Analysis via inhibition of transferrin recycling by low temperatures. J Biol Chem 266:3469-74
Sipe, D M; Murphy, R F (1991) Binding to cellular receptors results in increased iron release from transferrin at mildly acidic pH. J Biol Chem 266:8002-7
Roederer, M; Barry, J R; Wilson, R B et al. (1990) Endosomes can undergo an ATP-dependent density increase in the absence of dense lysosomes. Eur J Cell Biol 51:229-34
Bowser, R; Murphy, R F (1990) Kinetics of hydrolysis of endocytosed substrates by mammalian cultured cells: early introduction of lysosomal enzymes into the endocytic pathway. J Cell Physiol 143:110-7
Yamashiro, C T; Kane, P M; Wolczyk, D F et al. (1990) Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase. Mol Cell Biol 10:3737-49

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