Abstract

Site specific mutations in the metallothionein coding sequence will be followed for their expression. Selected codons will be specifically altered to give rise to amino acid replacement from non-aromatic to aromatic. The latter is normally absent in all native metallothioneins thus far analyzed. The possibility that the absence of aromatic amino acids is due to mutational probability of unfavorable processing at the transcriptional or translational level will be examined in vivo and in vitro. The altered metallothionein if obtained will be compared with the function and properties of those occurring naturally.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM032606-06
Application #
3281616
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1984-01-01
Project End
1991-12-31
Budget Start
1989-01-01
Budget End
1989-12-31
Support Year
6
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Public Health
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Cody, C W; Huang, P C (1994) Replacement of all alpha-domain lysines with glutamates reduces metallothionein detoxification function. Biochem Biophys Res Commun 202:954-9
Cody, C W; Huang, P C (1993) Metallothionein detoxification function is impaired by replacement of both conserved lysines with glutamines in the hinge between the two domains. Biochemistry 32:5127-31
Chernaik, M L; Huang, P C (1991) Differential effect of cysteine-to-serine substitutions in metallothionein on cadmium resistance. Proc Natl Acad Sci U S A 88:3024-8
Cismowski, M J; Narula, S S; Armitage, I M et al. (1991) Mutation of invariant cysteines of mammalian metallothionein alters metal binding capacity, cadmium resistance, and 113Cd NMR spectrum. J Biol Chem 266:24390-7
Cismowski, M J; Huang, P C (1991) Purification of mammalian metallothionein from recombinant systems. Methods Enzymol 205:312-9
Lin, L Y; Huang, P C (1990) Complete homology in metallothionein from two genera of ducks and their hybrids. Biochem Biophys Res Commun 168:182-7
Lin, L Y; Lin, W C; Huang, P C (1990) Pigeon metallothionein consists of two species. Biochim Biophys Acta 1037:248-55
Rhee, I K; Lee, K S; Huang, P C (1990) Metallothioneins with interdomain hinges expanded by insertion mutagenesis. Protein Eng 3:205-13
Lin, L Y; Liu, L F; Tam, M F et al. (1990) Primary sequence of duck metallothionein. Biochim Biophys Acta 1041:31-5
Morris, S; Huang, P C (1989) Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cdr variant. Exp Cell Res 185:166-75

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