Abstract

This proposal builds on the established library of monoclonal antibodies to Drosophila nuclear protein and the recently developed tools of three-dimensional reconstruction of subcellular structures. This proposal focusses on a subset of the antibodies that label the nuclear envelope. Using electron microscopy, the substructure and location within the nuclear envelope will be established. Further correlation of the nuclear envelope substructures to the interphase chromosomes by the three-dimensional image processing techniques will be accomplished. The antigen will be purified and extensively characterized by biochemical procedures.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM032803-03
Application #
3281936
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1983-12-01
Project End
1986-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Agard, D A; Hiraoka, Y; Shaw, P et al. (1989) Fluorescence microscopy in three dimensions. Methods Cell Biol 30:353-77
Minden, J S; Agard, D A; Sedat, J W et al. (1989) Direct cell lineage analysis in Drosophila melanogaster by time-lapse, three-dimensional optical microscopy of living embryos. J Cell Biol 109:505-16
Agard, D A; Hiraoka, Y; Sedat, J W (1988) Three-dimensional light microscopy of diploid Drosophila chromosomes. Cell Motil Cytoskeleton 10:18-27
Hiraoka, Y; Sedat, J W; Agard, D A (1987) The use of a charge-coupled device for quantitative optical microscopy of biological structures. Science 238:36-41