The nuclear envelope separating the major compartments of the eukaryotic cell breaks down at the beginning of mitosis and is reformed around the separated chromosomes at the end of mitosis. Breakdown and reconstruction must be regulated precisely. This proposal aims at elucidating: functions and structural changes that are dependent on and independent of a reconstructed nuclear envelope; the signals or factors that remain on the mitotic nuclear membranes; and possibly those proteins that are reassociating with the nuclear membranes during reconstruction. Technically, monoclonal antibodies against the membrane-chromatin connecting antigens will be microinjected and the cells observed ultrastructurally. Mitotic nuclear membranes will be obtained by affinity chromatography to study events of nuclear envelope reconstruction. These cell biological investigations will establish the framework for the future search for mitosis-inducing factors and their antagonists.
| Maul, G G; Schatten, G; Jimenez, S A et al. (1987) Detection of nuclear lamin B epitopes in oocyte nuclei from mice, sea urchins, and clams using a human autoimmune serum. Dev Biol 121:368-75 |
| French, B T; Maul, H M; Maul, G G (1986) Screening cDNA expression libraries with monoclonal and polyclonal antibodies using an amplified biotin-avidin-peroxidase technique. Anal Biochem 156:417-23 |
| Maul, G G; French, B T; Bechtol, K B (1986) Identification and redistribution of lamins during nuclear differentiation in mouse spermatogenesis. Dev Biol 115:68-77 |
| French, B T; Lee, W H; Maul, G G (1985) Nucleotide sequence of a cDNA clone for mouse pro alpha 1(I) collagen protein. Gene 39:311-2 |
