Abstract

This proposal is concerned with the mechanism of gene expression of mouse mitochondrial DNA. The ribosomal DNA region of this genome has been shown to be actively transcribed and the ribosomal RNA genes are located in close proximity to the origin of heavy strand DNA replication. We are utilizing cloned mitochondrial DNA fragments for hybridization and sequencing experiments combined with RNA sequencing to analyze this common control region for initiation of DNA replication and transcription.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM033088-18
Application #
3282444
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1979-02-01
Project End
1989-01-31
Budget Start
1988-02-01
Budget End
1989-01-31
Support Year
18
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Shadel, G S; Buckenmeyer, G A; Clayton, D A et al. (2000) Mutational analysis of the RNA component of Saccharomyces cerevisiae RNase MRP reveals distinct nuclear phenotypes. Gene 245:175-84
Shadel, G S; Clayton, D A (1997) Mitochondrial DNA maintenance in vertebrates. Annu Rev Biochem 66:409-35
Paluh, J L; Clayton, D A (1996) Mutational analysis of the gene for Schizosaccharomyces pombe RNase MRP RNA, mrp1, using plasmid shuffle by counterselection on canavanine. Yeast 12:1393-405
Paluh, J L; Clayton, D A (1996) A functional dominant mutation in Schizosaccharomyces pombe RNase MRP RNA affects nuclear RNA processing and requires the mitochondrial-associated nuclear mutation ptp1-1 for viability. EMBO J 15:4723-33
Jeong-Yu, S; Davis, A F; Clayton, D A (1996) Subtle determinants of the nucleocytoplasmic partitioning of in vivo-transcribed RNase MRP RNA in Xenopus laevis oocytes. Gene Expr 5:155-67
Carrodeguas, J A; Yun, S; Shadel, G S et al. (1996) Functional conservation of yeast mtTFB despite extensive sequence divergence. Gene Expr 6:219-30
Davis, A F; Ropp, P A; Clayton, D A et al. (1996) Mitochondrial DNA polymerase gamma is expressed and translated in the absence of mitochondrial DNA maintenance and replication. Nucleic Acids Res 24:2753-9
Lee, D Y; Clayton, D A (1996) Properties of a primer RNA-DNA hybrid at the mouse mitochondrial DNA leading-strand origin of replication. J Biol Chem 271:24262-9
Shadel, G S; Clayton, D A (1996) Mapping promoters in displacement-loop region of vertebrate mitochondrial DNA. Methods Enzymol 264:139-48
Xu, B; Clayton, D A (1996) RNA-DNA hybrid formation at the human mitochondrial heavy-strand origin ceases at replication start sites: an implication for RNA-DNA hybrids serving as primers. EMBO J 15:3135-43

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