The initial thrust of the proposed research is to use fluorescent double-stranded DNA to ingredients for synthesizing the requisite DNA derivatives are reaction of single-stranded DNA with chloroacetaldehyde and a thermal reannealing step. The known properties of recA protein dictate the nature of the DNA materials to be employed in these syntheses. Those materials should enable us to develop a novel method for monitoring strand transfer reactions continuously with both stopped-flow and steady-state fluorometers. The second major focus of the proposal is the utilization of fluorescent single-stranded DNA to test proposed mechanisms for recAp-catalyzed reactions. In all, the experiments suggested here should afford new insights into the mechanism of genetic recombination, one of the most fundamental biological processes.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM033651-03
Application #
3283549
Study Section
Biophysics and Biophysical Chemistry A Study Section (BBCA)
Project Start
1986-08-01
Project End
1990-07-31
Budget Start
1988-08-01
Budget End
1990-07-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Amherst College
Department
Type
Schools of Arts and Sciences
DUNS #
City
Amherst
State
MA
Country
United States
Zip Code
01002