Abstract

DNA molecules exchange parts with each other in a variety of circumstances. In meiosis, eukaryotic chromosomes break and join with strict adherance to homology, so that the order of gene loci is preserved. This process, generalized genetic recombination, will be further investigated in yeast (S. cerevisiae) and phage lambda. A fuller understanding of recombination may have implications for developmental anomolies, immune deficiencies and cancer induction. This continuing investigation will emphasize recombination initiated by double-chain breaks. Such breaks initiate both mitotic and meiotic exchange in yeast and recombination by the Red, RecE and RecF pathways operating on lambda. The RecBCD pathway is, likewise, dependent on double chain breaks, although the induced exchange is remote from the break. The molecular mechanisms involved will be elucidated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM033677-07
Application #
3283572
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1984-08-01
Project End
1992-07-31
Budget Start
1990-08-01
Budget End
1991-07-31
Support Year
7
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Oregon
Department
Type
Organized Research Units
DUNS #
948117312
City
Eugene
State
OR
Country
United States
Zip Code
97403

  Publications

Tarkowski, Trudee A; Mooney, Duane; Thomason, Lynn C et al. (2002) Gene products encoded in the ninR region of phage lambda participate in Red-mediated recombination. Genes Cells 7:351-63
Copenhaver, G P; Housworth, E A; Stahl, F W (2002) Crossover interference in Arabidopsis. Genetics 160:1631-9
Stahl, F; Bowers Jr, R; Mooney, D et al. (2001) Growth and recombination of phage lambda in the presence of exonuclease V from Bacillus subtilis. Mol Gen Genet 264:716-23
Stahl, F W; Hillers, K J (2000) Heteroduplex rejection in yeast? Genetics 154:1913-6
Hillers, K J; Stahl, F W (1999) The conversion gradient at HIS4 of Saccharomyces cerevisiae. I. Heteroduplex rejection and restoration of Mendelian segregation. Genetics 153:555-72
Thompson, D A; Stahl, F W (1999) Genetic control of recombination partner preference in yeast meiosis. Isolation and characterization of mutants elevated for meiotic unequal sister-chromatid recombination. Genetics 153:621-41
Foss, H M; Hillers, K J; Stahl, F W (1999) The conversion gradient at HIS4 of Saccharomyces cerevisiae. II. A role for mismatch repair directed by biased resolution of the recombinational intermediate. Genetics 153:573-83
Stahl, F W (1998) Recombination in phage lambda: one geneticist's historical perspective. Gene 223:95-102
Feher, V A; Zapf, J W; Hoch, J A et al. (1997) High-resolution NMR structure and backbone dynamics of the Bacillus subtilis response regulator, Spo0F: implications for phosphorylation and molecular recognition. Biochemistry 36:10015-25
Zhou, H; Dahlquist, F W (1997) Phosphotransfer site of the chemotaxis-specific protein kinase CheA as revealed by NMR. Biochemistry 36:699-710

Showing the most recent 10 out of 53 publications