Abstract

We are interested in using Saccharomyces cerevisiae as our model system to study chromosome replication in eukaryotes. By means of conventional genetics and current techniques developed in molecular cloning, we hope to dissect the complex chromosomes into simple replication units so as to: (1) study the structure, complexity and organization of individual replication initiation sites (replicators), and (2) examine the signals that activate replication at these sites. By gathering the information from these studies, we hope to learn something about the replication of chromosomes as a whole and the control mechanism which initiates their replication.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
9R01GM034190-07A1
Application #
3284759
Study Section
Genetics Study Section (GEN)
Project Start
1978-04-01
Project End
1987-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Cornell University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850

  Publications

Liachko, Ivan; Tye, Bik K (2005) Mcm10 is required for the maintenance of transcriptional silencing in Saccharomyces cerevisiae. Genetics 171:503-15
Douglas, Nancy L; Dozier, Samantha K; Donato, Justin J (2005) Dual roles for Mcm10 in DNA replication initiation and silencing at the mating-type loci. Mol Biol Rep 32:197-204
Sawyer, Sara L; Cheng, Irene H; Chai, Weihang et al. (2004) Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae. J Mol Biol 340:195-202
Chang, Victoria K; Donato, Justin J; Chan, Clarence S et al. (2004) Mcm1 promotes replication initiation by binding specific elements at replication origins. Mol Cell Biol 24:6514-24
Tye, Bik K; Chang, Victoria K (2004) Dual functional regulators coordinate DNA replication and gene expression in proliferating cells. Front Biosci 9:2548-55
Fitch, Michael J; Donato, Justin J; Tye, Bik K (2003) Mcm7, a subunit of the presumptive MCM helicase, modulates its own expression in conjunction with Mcm1. J Biol Chem 278:25408-16
Christensen, Tim W; Tye, Bik K (2003) Drosophila MCM10 interacts with members of the prereplication complex and is required for proper chromosome condensation. Mol Biol Cell 14:2206-15
Chang, Victoria K; Fitch, Michael J; Donato, Justin J et al. (2003) Mcm1 binds replication origins. J Biol Chem 278:6093-100
Lei, Ming; Cheng, Irene H; Roberts, Louis A et al. (2002) Two mcm3 mutations affect different steps in the initiation of DNA replication. J Biol Chem 277:30824-31
Yu, Xiong; VanLoock, Margaret S; Poplawski, Andrzej et al. (2002) The Methanobacterium thermoautotrophicum MCM protein can form heptameric rings. EMBO Rep 3:792-7

Showing the most recent 10 out of 36 publications