Abstract

Bacteriophage Phi6 contains three pieces of double-stranded RNA in its nucleocapsid. The pieces contain different genetic information but have similar termini.
The aim of this proposal is to determine how the packaging system is able to select one of each of the chromosomes. Specifically, the regions of the three chromosomes necessary for packaging will be identified. The order of packaging and the requirements for packaging in terms of amount of RNA will be determined. The studies will depend upon the use of cDNA clones of the Phi6 chromosomes. Chimeras and other constructions of the cDNA clones will be prepared and the packaging of the transcripts of the cDNA will be studied. In addition, cDNA clones of the large chromosome will be prepared. The products of this chromosome should form procapsids capable of packaging RNA. The nature of the mechanisms of selective packaging in viruses of segmented genomes is currently unknown. The elucidation of the mechanism for Phi6 will probably be relevant to packaging of other segmented genome viruses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034352-05
Application #
3285202
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1985-01-01
Project End
1989-12-31
Budget Start
1989-01-01
Budget End
1989-12-31
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Public Health Research Institute
Department
Type
DUNS #
City
Newark
State
NJ
Country
United States
Zip Code
07103

  Publications

Nemecek, Daniel; Qiao, Jian; Mindich, Leonard et al. (2012) Packaging accessory protein P7 and polymerase P2 have mutually occluding binding sites inside the bacteriophage 6 procapsid. J Virol 86:11616-24
Nemecek, Daniel; Cheng, Naiqian; Qiao, Jian et al. (2011) Stepwise expansion of the bacteriophage ?6 procapsid: possible packaging intermediates. J Mol Biol 414:260-71
Qiao, Xueying; Sun, Yang; Qiao, Jian et al. (2010) Interaction of a host protein with core complexes of bacteriophage phi6 to control transcription. J Virol 84:4821-5
Qiao, Xueying; Sun, Yang; Qiao, Jian et al. (2010) Characterization of Phi2954, a newly isolated bacteriophage containing three dsRNA genomic segments. BMC Microbiol 10:55
Qiao, Jian; Qiao, Xueying; Sun, Yang et al. (2010) Role of host protein glutaredoxin 3 in the control of transcription during bacteriophage Phi2954 infection. Proc Natl Acad Sci U S A 107:6000-4
Nemecek, Daniel; Heymann, J Bernard; Qiao, Jian et al. (2010) Cryo-electron tomography of bacteriophage phi6 procapsids shows random occupancy of the binding sites for RNA polymerase and packaging NTPase. J Struct Biol 171:389-96
Qiao, Xueying; Sun, Yang; Qiao, Jian et al. (2009) Temporal control of message stability in the life cycle of double-stranded RNA bacteriophage phi8. J Virol 83:633-9
Qiao, Xueying; Sun, Yang; Qiao, Jian et al. (2008) The role of host protein YajQ in the temporal control of transcription in bacteriophage Phi6. Proc Natl Acad Sci U S A 105:15956-60
Sen, Anindito; Heymann, J Bernard; Cheng, Naiqian et al. (2008) Initial location of the RNA-dependent RNA polymerase in the bacteriophage Phi6 procapsid determined by cryo-electron microscopy. J Biol Chem 283:12227-31
Farver, Ole; Chen, Ying; Fee, James A et al. (2006) Electron transfer among the CuA-, heme b- and a3-centers of Thermus thermophilus cytochrome ba3. FEBS Lett 580:3417-21

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