Abstract

During the tenure of this grant studies on the mechanism of action of a number of enzymes involved in procaryotic DNA replication will be continued. Particular emphasis will be placed on the mechanism of action of the DNA elongation enzymes involving E. coli DNA polymerase III, DNA elongation factors I, III and dna Z. Studies on the specificity of the action of dna G in the synthesis of oligonucleotide primers will be continued. We also plan to examine the mechanism of action of the phi X A protein and the control of phi X RFI replication. The replication of adenoviral DNA will be studied in vitro. The mechanism of initiation and DNA elongation will be examined. Studies will also be carried out on Vaccinia viral transcription and the role of auxiliary proteins on transcription. Included in these studies will be an analysis of reactions ocurring at ends of RNA chains. The latter projects will also be carried out with nuclear extracts of HeLa cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034559-02
Application #
3285807
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1984-07-01
Project End
1985-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Cho, Won-Ho; Kang, Young-Hoon; An, Yun-Young et al. (2013) Human Tim-Tipin complex affects the biochemical properties of the replicative DNA helicase and DNA polymerases. Proc Natl Acad Sci U S A 110:2523-7
Kang, Young-Hoon; Farina, Andrea; Bermudez, Vladimir P et al. (2013) Interaction between human Ctf4 and the Cdc45/Mcm2-7/GINS (CMG) replicative helicase. Proc Natl Acad Sci U S A 110:19760-5
Yardimci, Hasan; Wang, Xindan; Loveland, Anna B et al. (2012) Bypass of a protein barrier by a replicative DNA helicase. Nature 492:205-9
Chemnitz Galal, Wiebke; Pan, Miao; Giulian, Gary et al. (2012) Formation of dAMP-glycerol and dAMP-Tris derivatives by Thermococcus kodakaraensis DNA primase. J Biol Chem 287:16220-9
Kang, Young-Hoon; Galal, Wiebke Chemnitz; Farina, Andrea et al. (2012) Properties of the human Cdc45/Mcm2-7/GINS helicase complex and its action with DNA polymerase epsilon in rolling circle DNA synthesis. Proc Natl Acad Sci U S A 109:6042-7
Li, Zhuo; Pan, Miao; Santangelo, Thomas J et al. (2011) A novel DNA nuclease is stimulated by association with the GINS complex. Nucleic Acids Res 39:6114-23
Fu, Yu V; Yardimci, Hasan; Long, David T et al. (2011) Selective bypass of a lagging strand roadblock by the eukaryotic replicative DNA helicase. Cell 146:931-41
Ladner, Jane E; Pan, Miao; Hurwitz, Jerard et al. (2011) Crystal structures of two active proliferating cell nuclear antigens (PCNAs) encoded by Thermococcus kodakaraensis. Proc Natl Acad Sci U S A 108:2711-6
Im, Jun-Sub; Ki, Sang-Hee; Farina, Andrea et al. (2009) Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci U S A 106:15628-32
Vijayakumar, Sangeetha; Dziegielewska, Barbara; Levin, David S et al. (2009) Phosphorylation of human DNA ligase I regulates its interaction with replication factor C and its participation in DNA replication and DNA repair. Mol Cell Biol 29:2042-52

Showing the most recent 10 out of 70 publications