Abstract

The division cycle of yeast a cells is inhibited by the peptide pheromone, alpha-factor. After prolonged exposure to alpha- factor, a cells """"""""adapt"""""""" to the pheromone and resume cell division. The proposed research examines the role of alpha-factor receptors in the control of these processes. Detailed understanding of this very basic process in yeast should provide information for a more general understanding of receptor action and cell division control in other organisms as well. A genetic approach will be applied to this problem. Mutants will be isolated which alter the responsiveness of a cells to alpha-factor. Defects in the processes that control receptor activity will be evaluated by performing pheromone binding and immunochemical assays. The control of cell division by polypeptide hormones has obvious medical significance.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034719-05
Application #
3286204
Study Section
Genetics Study Section (GEN)
Project Start
1985-04-01
Project End
1993-03-31
Budget Start
1989-04-01
Budget End
1990-03-31
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Massachusetts Medical School Worcester
Department
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655

  Publications

Dosil, M; Schandel, K A; Gupta, E et al. (2000) The C terminus of the Saccharomyces cerevisiae alpha-factor receptor contributes to the formation of preactivation complexes with its cognate G protein. Mol Cell Biol 20:5321-9
Yesilaltay, A; Jenness, D D (2000) Homo-oligomeric complexes of the yeast alpha-factor pheromone receptor are functional units of endocytosis. Mol Biol Cell 11:2873-84
Hirschman, J E; Jenness, D D (1999) Dual lipid modification of the yeast ggamma subunit Ste18p determines membrane localization of Gbetagamma. Mol Cell Biol 19:7705-11
Li, Y; Kane, T; Tipper, C et al. (1999) Yeast mutants affecting possible quality control of plasma membrane proteins. Mol Cell Biol 19:3588-99
Hirschman, J E; De Zutter, G S; Simonds, W F et al. (1997) The G beta gamma complex of the yeast pheromone response pathway. Subcellular fractionation and protein-protein interactions. J Biol Chem 272:240-8
Bukusoglu, G; Jenness, D D (1996) Agonist-specific conformational changes in the yeast alpha-factor pheromone receptor. Mol Cell Biol 16:4818-23
Song, J; Hirschman, J; Gunn, K et al. (1996) Regulation of membrane and subunit interactions by N-myristoylation of a G protein alpha subunit in yeast. J Biol Chem 271:20273-83
Schandel, K A; Jenness, D D (1994) Direct evidence for ligand-induced internalization of the yeast alpha-factor pheromone receptor. Mol Cell Biol 14:7245-55
Hasson, M S; Blinder, D; Thorner, J et al. (1994) Mutational activation of the STE5 gene product bypasses the requirement for G protein beta and gamma subunits in the yeast pheromone response pathway. Mol Cell Biol 14:1054-65
Konopka, J B; Jenness, D D (1991) Genetic fine-structural analysis of the Saccharomyces cerevisiae alpha-pheromone receptor. Cell Regul 2:439-52

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