Nucleosome assembly will studied in vitro using extracts of HeLa cell nuclei as a source of somatic cell nucleosome assembly factors. The appropriate DNA, histone modification state, incubation conditions will be determined for study of in vitro assembly. The formation of pre-nucleosomal histone-histone complexes, histone-DNA complexes, and factor-DNA complexes will be examined in order to establish the assembly pathway. Chromatin assembled in vitro will be tested for its ability to be transcribed by injection into Xenopus oocytes. Incubation of chromatin extracts derived from cells actively expressing sequences to be tested will be performed before, during, and after assembly in order to study the formation of transcriptionally active chromatin.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034808-03
Application #
3286409
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1984-09-01
Project End
1989-02-28
Budget Start
1986-03-01
Budget End
1987-02-28
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Zymogenetics, Inc.
Department
Type
DUNS #
City
Seattle
State
WA
Country
United States
Zip Code
98105