Abstract

A major focus of our studies will be the regulation of transcription and of stability of mRNAs specific for prespore and prestalk cells, as well as mRNAs synthesized throughout growth and differentiation. These studies will include mapping and sequencing of genomic DNA clones; in vitro studies of regulated mRNA synthesis and destruction; in situ detection of mRNAs; and determination of the commitment of prespore and prestalk cells. We will attempt to elucidate the intracellular """"""""second messenger(s)"""""""" that mediate the effect of the extracellular hormone, cAMP, on synthesis and stability of regulated mRNAs; and to detect specific cell surface proteins that are required for induction of gene expression in multicellular aggregates. Finally, we shall study the structure and regulation of transcription and mRNA processing of a class of repetitive DNA sequences that is expressed as mRNA only during differentiation of heat shock.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM035011-04
Application #
3287067
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1984-07-01
Project End
1988-12-31
Budget Start
1987-01-01
Budget End
1987-12-31
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Whitehead Institute for Biomedical Research
Department
Type
DUNS #
076580745
City
Cambridge
State
MA
Country
United States
Zip Code
02142

  Publications

Kopito, R R; Andersson, M M; Herzlinger, D A et al. (1988) Structure and tissue-specific expression of the mouse anion-exchanger gene in erythroid and renal cells. Soc Gen Physiol Ser 43:151-61
Kopito, R R; Andersson, M A; Lodish, H F (1987) Multiple tissue-specific sites of transcriptional initiation of the mouse anion antiport gene in erythroid and renal cells. Proc Natl Acad Sci U S A 84:7149-53
Amara, J F; Lodish, H F (1987) Specific mRNA destabilization in Dictyostelium discoideum requires RNA synthesis. Mol Cell Biol 7:4585-8
Chisholm, R L; Hopkinson, S; Lodish, H F (1987) Superinduction of the Dictyostelium discoideum cell surface cAMP receptor by pulses of cAMP. Proc Natl Acad Sci U S A 84:1030-4
Kopito, R R; Andersson, M; Lodish, H F (1987) Structure and organization of the murine band 3 gene. J Biol Chem 262:8035-40
Cappello, J; Handelsman, K; Lodish, H F (1985) Sequence of Dictyostelium DIRS-1: an apparent retrotransposon with inverted terminal repeats and an internal circle junction sequence. Cell 43:105-15