Agents that damage DNA reduce viability and can be mutagenic and carcinogenic. The long-term goals of this project are to understand cellular responses to DNA damage. In bacteria, the RecA protein controls the synthesis of enzymes that repair DNA and also directly participates in several repair pathways. To study the synthesis of RecA, mutants from a collection that affect its expression will be analyzed. To study the action of RecA, mutants that alter important sites in the protein will be studied. The possibility that some of the functions of RecA require its export from the cytoplasm will be studied with protein fusions to alkaline phosphatase. The relation of the amount of RecA in the cell to its activities will be determined using a hybrid gene in which RecA is made using the lac promoter. Changes in intracellular metabolism will also be studied by following the expression of cea, the gene for colicin E1, which is controlled by RecA, catabolite repression, anaerobiosis, nutrient deprivation, and DNA supercoiling. DNA rearrangements that result from recombination between repeated sequences will also be analyzed after DNA damaging treatments to determine if there may be constraints on recombination to maintain proper chromosome structure.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM035247-07
Application #
3287662
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1985-08-30
Project End
1993-07-31
Budget Start
1991-08-01
Budget End
1993-07-31
Support Year
7
Fiscal Year
1991
Total Cost
Indirect Cost
Name
University of Texas Health Science Center Houston
Department
Type
Schools of Medicine
DUNS #
City
Houston
State
TX
Country
United States
Zip Code
77225
Perkins, J D; Heath, J D; Sharma, B R et al. (1993) XbaI and BlnI genomic cleavage maps of Escherichia coli K-12 strain MG1655 and comparative analysis of other strains. J Mol Biol 232:419-45
Eraso, J M; Weinstock, G M (1992) Anaerobic control of colicin E1 production. J Bacteriol 174:5101-9
Perkins, J D; Heath, J D; Sharma, B R et al. (1992) SfiI genomic cleavage map of Escherichia coli K-12 strain MG1655. Nucleic Acids Res 20:1129-37
Heath, J D; Perkins, J D; Sharma, B et al. (1992) NotI genomic cleavage map of Escherichia coli K-12 strain MG1655. J Bacteriol 174:558-67
Heath, J D; Weinstock, G M (1991) Tandem duplications of the lac region of the Escherichia coli chromosome. Biochimie 73:343-52
Weisemann, J M; Weinstock, G M (1991) The promoter of the recA gene of Escherichia coli. Biochimie 73:457-70
Salles, B; Weinstock, G M (1989) Interaction of the CRP-cAMP complex with the cea regulatory region. Mol Gen Genet 215:537-42
Salles, B; Weinstock, G M (1989) Mutation of the promoter and LexA binding sites of cea, the gene encoding colicin E1. Mol Gen Genet 215:483-9
Weisemann, J M; Weinstock, G M (1988) Mutations at the cysteine codons of the recA gene of Escherichia coli. DNA 7:389-98
Salles, B; Weisemann, J M; Weinstock, G M (1987) Temporal control of colicin E1 induction. J Bacteriol 169:5028-34