The interactions between the tomato plant (Lycopersicon esculentum) and its pathogen, Pseudomonas syringae pv. tomato, provide an excellent system for the study of plant gene expression during host-pathogen interactions. Genes which are expressed in a disease-resistant plant (""""""""defense"""""""" genes) during bacterial pathogen infection will be isolated, and their structure and gene expression programs will be determined. The sequences which are important in the regulation of """"""""defense"""""""" gene expression will be identified by deletion mutant and gene fusion analysis. Intact and modified genes will be introduced into the disease-sensitive tomato genome; transformed plants will be regenerated and analyzed for the structure, organization, and expression of the """"""""defense"""""""" gene. In addition, transformed plants will be tested for the expression of the disease-resistance phenotype. These experiments will provide new and basic information about how plant gene expression is regulated during a biological stress in a higher eukaryotic.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM035928-03
Application #
3289375
Study Section
Genetics Study Section (GEN)
Project Start
1986-09-01
Project End
1990-08-31
Budget Start
1988-09-01
Budget End
1990-08-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of California Riverside
Department
Type
Earth Sciences/Resources
DUNS #
City
Riverside
State
CA
Country
United States
Zip Code
92521
Pautot, V; Holzer, F M; Reisch, B et al. (1993) Leucine aminopeptidase: an inducible component of the defense response in Lycopersicon esculentum (tomato). Proc Natl Acad Sci U S A 90:9906-10
Pautot, V; Holzer, F M; Walling, L L (1991) Differential expression of tomato proteinase inhibitor I and II genes during bacterial pathogen invasion and wounding. Mol Plant Microbe Interact 4:284-92