The proposed project will determine structural elements for the enzyme responsible for nitrite reduction in dissimilatory denitrification. Proton nuclear magnetic resonance spectroscopy will be used to determine structure about the catalytic site heme groups, including the characterization of electronic states. Resonance raman spectroscopy will be used to investigate the vibrational states of the catalytic sites. The exact chemical structure of the prosthetic groups of the enzyme will be determined by purification of these from the intact enzyme and a structural determination through nuclear magnetic resonance, vibrational, optical, and mass spectroscopy. The interactions of oxides of nitrogen with whole cells will also be examined. The target enzyme system is representative of the general class of electron transport enzymes and proteins. Its study will promote the understanding on a fundamental level of this class which is responsible for energy production and respiration in all cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036264-05
Application #
3289891
Study Section
Biochemistry Study Section (BIO)
Project Start
1985-07-01
Project End
1991-04-30
Budget Start
1989-05-01
Budget End
1991-04-30
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Alabama in Tuscaloosa
Department
Type
Schools of Arts and Sciences
DUNS #
City
Tuscaloosa
State
AL
Country
United States
Zip Code
35487
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