The overall long-term objective of the proposed is to increase our understanding of the population genetics and modes of evolution of mobile elements in eukaryotes using the P element family as a model system. P elements are one of two transposable element families which are associated with the hybrid dysgenesis syndrome in Drosophila melanogaster and they have many similarities with mobile elements in other higher organisms such as maize.
Specific aims are to obtain a thorough understanding of the relationship between the functional and molecular properties of P elements and to determine the extent and nature of the effects of P element sequences on the phenotype of the Drosophila organism. The dynamics of P elements will be investigated in both natural and laboratory populations. The rate and direction of their evolution will be determined in newly invaded populations and factors affecting their regulation will be studied. As essentially experimental approach will be adopted to achieve these goals. Both genetic and biochemical methods will be used to determine the functional and molecular properties of P elements respectively. Informal collaboration with other laboratories employing specialized molecular genetic techniques is planned. With the growing realization of the ubiquity of transposable elements in higher organisms and their role in the induction of mutations, chromosomal abberations and genomic reorganization, there is considerable potential for the application of basic research on transposable elements to various aspects of human health.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036715-02
Application #
3291177
Study Section
Genetics Study Section (GEN)
Project Start
1985-09-01
Project End
1989-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Arizona
Department
Type
Schools of Arts and Sciences
DUNS #
City
Tucson
State
AZ
Country
United States
Zip Code
85722
de Frutos, R; Peterson, K R; Kidwell, M G (1992) Distribution of Drosophila melanogaster transposable element sequences in species of the obscura group. Chromosoma 101:293-300
Boussy, I A; Daniels, S B (1991) hobo transposable elements in Drosophila melanogaster and D. simulans. Genet Res 58:27-34
Daniels, S B; Chovnick, A; Boussy, I A (1990) Distribution of hobo transposable elements in the genus Drosophila. Mol Biol Evol 7:589-606
Engels, W R; Preston, C R; Thompson, P et al. (1989) In situ hybridization of Drosophila polytene chromosomes with digoxigenin-dUTP-labeled probes. Trends Genet 5:366
Anxolabehere, D; Kidwell, M G; Periquet, G (1988) Molecular characteristics of diverse populations are consistent with the hypothesis of a recent invasion of Drosophila melanogaster by mobile P elements. Mol Biol Evol 5:252-69
Kidwell, M G; Kimura, K; Black, D M (1988) Evolution of hybrid dysgenesis potential following P element contamination in Drosophila melanogaster. Genetics 119:815-28
Boussy, I A; Healy, M J; Oakeshott, J G et al. (1988) Molecular analysis of the P-M gonadal dysgenesis cline in eastern Australian Drosophila melanogaster. Genetics 119:889-902
Boussy, I A; Kidwell, M G (1987) The P-M hybrid dysgenesis cline in Eastern Australian Drosophila melanogaster: discrete P, Q and M regions are nearly contiguous. Genetics 115:737-45
Daniels, S B; Clark, S H; Kidwell, M G et al. (1987) Genetic transformation of Drosophila melanogaster with an autonomous P element: phenotypic and molecular analyses of long-established transformed lines. Genetics 115:711-23
Black, D M; Jackson, M S; Kidwell, M G et al. (1987) KP elements repress P-induced hybrid dysgenesis in Drosophila melanogaster. EMBO J 6:4125-35