Abstract

The research involves two major projects.
The aim of one project is to determine the number, gross structure, and chromosomal locations in normal rat cells and in rat pituitary tumor cells (GH-cells) of the genes for the pituitary hormones, prolactin, growth hormone, and the alpha and beta subunits of thyrotropin. We are currently screening a rat genomic library for recombinant lambda bacteriophages containing these genes. These hybrid bacteriophages will then be employed as probes for chromosomal localization by in situ hybridization.
The aim of the other project is to determine the primary site(s) at which thyroid and glucocorticoid hormones act to increase growth hormone synthesis by GH-cells, and to investigate the role of insulin in the synergistic regulation by these two types of hormones of growth hormone gene expression. A recombinant bacterial plasmid containing a DNA sequence coding for rat growth hormone which we have prepared will be employed as a hybridization probe in these studies to follow nuclear and cytoplasmic growth hormone-specific RNA sequences during hormonal induction.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM036847-01
Application #
3291407
Study Section
Endocrinology Study Section (END)
Project Start
1986-01-01
Project End
1990-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Mount Sinai School of Medicine
Department
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10029

  Publications

Fliss, M S; Hinkle, P M; Bancroft, C (1999) Expression cloning and characterization of PREB (prolactin regulatory element binding), a novel WD motif DNA-binding protein with a capacity to regulate prolactin promoter activity. Mol Endocrinol 13:644-57
Gaiddon, C; Mercken, L; Bancroft, C et al. (1995) Transcriptional effects in GH3 cells of Gs alpha mutants associated with human pituitary tumors: stimulation of adenosine 3',5'-monophosphate response element-binding protein-mediated transcription and of prolactin and growth hormone promoter activity via Endocrinology 136:4331-8
Tian, J; Ma, H W; Bancroft, C (1995) Constitutively active Gq-alpha stimulates prolactin promoter activity via a pathway involving Raf activity. Mol Cell Endocrinol 112:249-56
Tian, J; Chen, J; Bancroft, C (1994) Expression of constitutively active Gs alpha-subunits in GH3 pituitary cells stimulates prolactin promoter activity. J Biol Chem 269:33-6
Yan, G; Chen, X; Bancroft, C (1994) A constitutively active form of CREB can activate expression of the rat prolactin promoter in non-pituitary cells. Mol Cell Endocrinol 101:R25-30
Fischberg, D J; Chen, X H; Bancroft, C (1994) A Pit-1 phosphorylation mutant can mediate both basal and induced prolactin and growth hormone promoter activity. Mol Endocrinol 8:1566-73
Coleman, D T; Bancroft, C (1993) Pituitary adenylate cyclase-activating polypeptide stimulates prolactin gene expression in a rat pituitary cell line. Endocrinology 133:2736-42
Morris, A E; Kloss, B; McChesney, R E et al. (1992) An alternatively spliced Pit-1 isoform altered in its ability to trans-activate. Nucleic Acids Res 20:1355-61
McChesney, R; Sealfon, S C; Tsutsumi, M et al. (1991) Either isoform of the dopamine D2 receptor can mediate dopaminergic repression of the rat prolactin promoter. Mol Cell Endocrinol 79:R1-7
Yan, G Z; Bancroft, C (1991) Mediation by calcium of thyrotropin--releasing hormone action on the prolactin promoter via transcription factor pit-1. Mol Endocrinol 5:1488-97

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