Abstract

The proposed research is aimed at dissecting the physiological processes that are required for IL2-driven proliferation of a murine cloned T cell line, L2. This entails the characterization of pH and volume regulatory mechanisms and assessment of membrane conductance. The primary approaches used to investigate these research areas are I9F NMR, electronic cell sizing and patch-clamp methods. Quiescent and IL2-stimulated L2 cells will be characterized with respect to each of the aforementioned physiological parameters. Additionally, these parameters will be assessed during specific phases of the cell division cycle.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036962-05
Application #
3291715
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1986-07-01
Project End
1991-06-30
Budget Start
1990-07-01
Budget End
1991-06-30
Support Year
5
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Clevenger, C V; Sillman, A L; Hanley-Hyde, J et al. (1992) Requirement for prolactin during cell cycle regulated gene expression in cloned T-lymphocytes. Endocrinology 130:3216-22
Clevenger, C V; Altmann, S W; Prystowsky, M B (1991) Requirement of nuclear prolactin for interleukin-2--stimulated proliferation of T lymphocytes. Science 253:77-9
Sabath, D E; Broome, H E; Prystowsky, M B (1990) Glyceraldehyde-3-phosphate dehydrogenase mRNA is a major interleukin 2-induced transcript in a cloned T-helper lymphocyte. Gene 91:185-91
Clevenger, C V; Sillman, A L; Prystowsky, M B (1990) Interleukin-2 driven nuclear translocation of prolactin in cloned T-lymphocytes. Endocrinology 127:3151-9
Sabath, D E; Podolin, P L; Comber, P G et al. (1990) cDNA cloning and characterization of interleukin 2-induced genes in a cloned T helper lymphocyte. J Biol Chem 265:12671-8
Lee, S C; Price, M; Prystowsky, M B et al. (1988) Volume response of quiescent and interleukin 2-stimulated T-lymphocytes to hypotonicity. Am J Physiol 254:C286-96
Shipman, P M; Sabath, D E; Fischer, A H et al. (1988) Cyclin mRNA and protein expression in recombinant interleukin 2-stimulated cloned murine T lymphocytes. J Cell Biochem 38:189-98
Moore, K S; Sullivan, K; Tan, E M et al. (1987) Proliferating cell nuclear antigen/cyclin is an interleukin 2-responsive gene. J Biol Chem 262:8447-50