Abstract

We wish to study a number of proteins relevant to the regulation of mitochondrial motility and bioenergetics in living cells. The goal of this proposal includes: 1. Identification of mitochondrial outer membrane proteins that interact with microtubules; 2. Identification of mitochondrial outer membrane proteins that interact with intermediate filaments; 3. Microtubule associated proteins that bind mitochondria; 4. Intermediate filaments associated proteins that bind mitochondria; 5. Novel cellular framework proteins that bind mitochondria; 6. Regulation of mitochondrial morphology in living cells; 7. Regulation of mitochondrial electrochemical gradient in living cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM038318-02
Application #
3294666
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1987-04-01
Project End
1992-03-31
Budget Start
1988-04-01
Budget End
1989-03-31
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Weber, G F; Daley, J; Kraeft, S K et al. (1997) Measurement of apoptosis in heterogenous cell populations. Cytometry 27:136-44
Mannion, B A; Berditchevski, F; Kraeft, S K et al. (1996) Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). J Immunol 157:2039-47
Sun, X; Wong, J R; Song, K et al. (1996) Anticarcinoma activity of a novel drug, 3-ethyl-3'-methyl-thiatelluracarbocyanine iodide (Te), a tellurium-containing cyanine targeted at mitochondria. Clin Cancer Res 2:1335-40
Wong, J R; Ho, C; Mauch, P et al. (1995) Removal of carcinoma cells from contaminated bone marrow using the lipophilic cation rhodamine 123. Clin Cancer Res 1:621-30
Lee, C; Wu, S S; Chen, L B (1995) Photosensitization by 3,3'-dihexyloxacarbocyanine iodide: specific disruption of microtubules and inactivation of organelle motility. Cancer Res 55:2063-9
Kuznetsov, G; Chen, L B; Nigam, S K (1994) Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. J Biol Chem 269:22990-5
Lo, S H; Janmey, P A; Hartwig, J H et al. (1994) Interactions of tensin with actin and identification of its three distinct actin-binding domains. J Cell Biol 125:1067-75
Lo, S H; An, Q; Bao, S et al. (1994) Molecular cloning of chick cardiac muscle tensin. Full-length cDNA sequence, expression, and characterization. J Biol Chem 269:22310-9
McGlade, J; Brunkhorst, B; Anderson, D et al. (1993) The N-terminal region of GAP regulates cytoskeletal structure and cell adhesion. EMBO J 12:3073-81
Davis, S; Lu, M L; Lo, S H et al. (1991) Presence of an SH2 domain in the actin-binding protein tensin. Science 252:712-5

Showing the most recent 10 out of 17 publications