The yeast pheromone response provides an excellent model both for G protein mediated signalling as well as adaptation. We propose to use a combination of genetic and molecular approaches to identify and characterize elements involved in both processes. Mutational analysis will be used to perform a structure/function analysis of the subunits of the G protein. Assays involving guanyl nucleotide metabolism and subunit interaction will be established for this purpose. A number of genetic screens will be employed to identify elements in both the signalling and adaptive pathways. It is hoped that the effectors for these pathways can be identified via these screens. Once genes encoding signaling elements have been identified, recombinant DNA methods will be used to express them in bacteria so that immunogenic quantities can be purified for antibody production. These antibodies will serve as reagents for cytological and biochemical studies of the signalling elements. The intention of this research is to provide a comprehensive description of cell surface receptor mediated signalling from its inception at the plasmid membrane to the effector pathway ultimately responsible for controlling gene expression.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM039429-07
Application #
2179813
Study Section
Genetics Study Section (GEN)
Project Start
1988-01-20
Project End
1994-12-31
Budget Start
1994-01-01
Budget End
1994-12-31
Support Year
7
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037