Abstract

Dr. Fukui is interested in studying how the actomyosin cytoskeleton is spatially and temporally organized in the cytoplasm. To pursue these problems, he will employ Dictyostelium discoideum. In this proposal, he addresses: (1) movement and assembly of myosin, in real time, in the cytoplasm of living cells; (2) quantification of vesicle movement in living cells; (3) tactile response of individual cells and the force generation of surface projections; and (4) the organizing center of the actomyosin cytoskeleton. Methods include video microscopy of micro- injected fluroescence-labeled myosin, of the vesicle movement, micro- manipulation of surface projections, and force measurement. He will also study the organization of myosin filaments with rapid freeze and rapid dry electron microscopy. He wishes to study both wild type and genetically engineered mutants.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM039548-07
Application #
2179904
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1988-12-01
Project End
1997-11-30
Budget Start
1994-12-01
Budget End
1995-11-30
Support Year
7
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
Northwestern University at Chicago
Department
Anatomy/Cell Biology
Type
Schools of Dentistry
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611

  Publications

Fukui, Yoshio (2002) Mechanistics of amoeboid locomotion: signal to forces. Cell Biol Int 26:933-44
Fukui, Y; de Hostos, E; Yumura, S et al. (1999) Architectural dynamics of F-actin in eupodia suggests their role in invasive locomotion in Dictyostelium. Exp Cell Res 249:33-45
Fukui, Y; Engler, S; Inoue, S et al. (1999) Architectural dynamics and gene replacement of coronin suggest its role in cytokinesis. Cell Motil Cytoskeleton 42:204-17
Fukui, Y; Kitanishi-Yumura, T; Yumura, S (1999) Myosin II-independent F-actin flow contributes to cell locomotion in dictyostelium. J Cell Sci 112 ( Pt 6):877-86
Yumura, S; Fukui, Y (1998) Spatiotemporal dynamics of actin concentration during cytokinesis and locomotion in Dictyostelium. J Cell Sci 111 ( Pt 15):2097-108
Aizawa, H; Fukui, Y; Yahara, I (1997) Live dynamics of Dictyostelium cofilin suggests a role in remodeling actin latticework into bundles. J Cell Sci 110 ( Pt 19):2333-44
Fukui, Y; Inoue, S (1997) Amoeboid movement anchored by eupodia, new actin-rich knobby feet in Dictyostelium. Cell Motil Cytoskeleton 36:339-54
Chu, Q; Fukui, Y (1996) In vivo dynamics of myosin II in Dictyostelium by fluorescent analogue cytochemistry. Cell Motil Cytoskeleton 35:254-68
Morita, Y S; Jung, G; Hammer 3rd, J A et al. (1996) Localization of Dictyostelium myoB and myoD to filopodia and cell-cell contact sites using isoform-specific antibodies. Eur J Cell Biol 71:371-9
Fukui, Y; Inoue, S (1995) Chemotaxis, aggregation behavior, and foot formation in Dictyostelium discoideum amoeba controlled by microbeam uncaging of cyclic-AMP. Biol Bull 189:198-9

Showing the most recent 10 out of 15 publications