A new method of focusing x-rays is described using appropriately tapered capillaries. The x-rays are incident on the inner surface of the capillary below the critical glancing angle and reflect due to total external reflection. By appropriately narrowing the capillary, the x-rays can thus be focused in a broad band of energies. The theory of the effect and optimum taper is described. A measurement verifying the focusing capability of the method is presented. The method appears practical for focusing bending magnet synchrotron radiation around 2 keV down to a diameter of 10 mu m from an initial dimension 1 mm2 incident cross section with an attenuation of the total energy of about 2, i.e., an increase in the intensity per unit area of 6.5 x 103. Greater focusing is possible with softer x-rays and from undulator sources. The technique has wide-ranging applicability. This proposal requests funding to develop this new methodology and to apply it in conjunction with flow methods in order to obtain kinetic x-ray absorption fine structure (XAFS) data of excited molecular states and dynamic protein conformational states with um sec time resolution.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM039803-02
Application #
3297009
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1988-04-01
Project End
1991-03-31
Budget Start
1989-04-01
Budget End
1990-03-31
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
Schools of Arts and Sciences
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Costa, C; Moura, J J; Moura, I et al. (1996) Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. J Biol Chem 271:23191-6