Abstract

The primary objectives of this project are to characterize multivalent cation binding environments on DNA and to explore how these environments change over the course of a biologically important DNA structural transition, the B-Z transition. Specifically, a series of 59 Co NMR measurements are proposed in order (l) to clarify the 59 Co NMR relaxation behavior of Co (NH3)63+ bound to DNA, in particular with respect to the influence of cation mobility on the surface of the DNA (2) to examine base composition, sequence and DNA length effects on Co(NH3)63+ binding strength and heterogeneity and (3) to monitor the state of Co(NH3)63+ over the B-Z transition of synthetic DNA. Additional experiments are proposed using 13C and 15N NMR measurements in order (1) to characterize the dilute-solution behavior of specifically labeled polyamines (2) to determine the local pKa's of polyamines bound to DNA (3) to characterize polyamine binding to natural, synthetic and oligomeric DNA's (4) to examine the mobility of polyamines trapped near the DNA surface and (5) to probe polyamine involvement in the B-Z transition.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
7R01GM040438-03
Application #
3297962
Study Section
Molecular and Cellular Biophysics Study Section (BBCA)
Project Start
1990-09-01
Project End
1992-08-31
Budget Start
1990-09-01
Budget End
1992-08-31
Support Year
3
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Nebraska Lincoln
Department
Type
Schools of Arts and Sciences
DUNS #
555456995
City
Lincoln
State
NE
Country
United States
Zip Code
68588

  Publications

Xian, W; Tang, J X; Janmey, P A et al. (1999) The polyelectrolyte behavior of actin filaments: a 25Mg NMR study. Biochemistry 38:7219-26
Deng, H; Braunlin, W H (1996) Kinetics of sodium ion binding to DNA quadruplexes. J Mol Biol 255:476-83
Xu, Q; Jampani, S R; Deng, H et al. (1995) Chiral recognition of deoxyoligonucleotides by delta- and lambda-tris(ethylenediamine)cobalt(III). Biochemistry 34:14059-65
Deng, H; Braunlin, W H (1995) Duplex to quadruplex equilibrium of the self-complementary oligonucleotide d(GGGGCCCC). Biopolymers 35:677-81
Xu, Q; Shoemaker, R K; Braunlin, W H (1993) Induction of B-A transitions of deoxyoligonucleotides by multivalent cations in dilute aqueous solution. Biophys J 65:1039-49
Xu, Q; Deng, H; Braunlin, W H (1993) Selective localization and rotational immobilization of univalent cations on quadruplex DNA. Biochemistry 32:13130-7
Xu, Q; Jampani, S R; Braunlin, W H (1993) Rotational dynamics of hexaamminecobalt(III) bound to oligomeric DNA: correlation with cation-induced structural transitions. Biochemistry 32:11754-60
Braunlin, W H; Drakenberg, T; Nordenskiold, L (1992) Ca2+ binding environments on natural and synthetic polymeric DNA's. J Biomol Struct Dyn 10:333-43
Braunlin, W H; Xu, Q (1992) Hexaamminecobalt(III) binding environments on double-helical DNA. Biopolymers 32:1703-11
Berggren, E; Nordenskiold, L; Braunlin, W H (1992) Interpretation of 25Mg spin relaxation in Mg-DNA solutions: temperature variation and chemical exchange effects. Biopolymers 32:1339-50

Showing the most recent 10 out of 11 publications