The objective of this proposal is to further our understanding of the biology and regulation of P element, a transposable element found in Drosophila melanogaster. P elements are particularly interesting because their transposition is both genetically regulated (the P-M hybrid dygenesis syndrome) and tissue specific (transposition only occurs in the germline and not in somatic tissues). Recently we have shown that the germline specificity of P element transposition is regulated at the level of mRNA splicing. As a continuation of these studies we will: (1) Determine to the nucleotide which cis-acting sequences of the P element pre-mRNA transcript are required for the germline specificity. (2) Determine the spatial and developmental profile of the P element germline specific splice using a beta gal staining assay. (3) Genetically screen for mutants in the gene encoding the germline specific RNA splicing factor. (4) Attempt to isolate and maintain a Drosophila germline cell line that is capable of splicing the P element germline specific intron. (5) Attempt to establish an in vitro splicing system that will allow the biochemical identification and purification of the germline specific RNA splicing factor. (6) Identify which protein product(s) of P element encode the P cytotype repressor, (7) Determine the mechanism by which the P cytotype repressor functions.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM040451-05
Application #
3297988
Study Section
Genetics Study Section (GEN)
Project Start
1988-07-01
Project End
1993-06-30
Budget Start
1992-07-01
Budget End
1993-06-30
Support Year
5
Fiscal Year
1992
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Type
Schools of Arts and Sciences
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
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