Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM041269-08
Application #
2180756
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1989-07-01
Project End
1998-06-30
Budget Start
1996-07-01
Budget End
1997-06-30
Support Year
8
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Physiology
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Francis, S H; Turko, I V; Corbin, J D (2001) Cyclic nucleotide phosphodiesterases: relating structure and function. Prog Nucleic Acid Res Mol Biol 65:1-52
Francis, S H; Turko, I V; Grimes, K A et al. (2000) Histidine-607 and histidine-643 provide important interactions for metal support of catalysis in phosphodiesterase-5. Biochemistry 39:9591-6
Fink, T L; Francis, S H; Beasley, A et al. (1999) Expression of an active, monomeric catalytic domain of the cGMP-binding cGMP-specific phosphodiesterase (PDE5). J Biol Chem 274:34613-20
Corbin, J D; Francis, S H (1999) Cyclic GMP phosphodiesterase-5: target of sildenafil. J Biol Chem 274:13729-32
Turko, I V; Ballard, S A; Francis, S H et al. (1999) Inhibition of cyclic GMP-binding cyclic GMP-specific phosphodiesterase (Type 5) by sildenafil and related compounds. Mol Pharmacol 56:124-30
Francis, S H; Chu, D M; Thomas, M K et al. (1998) Ligand-induced conformational changes in cyclic nucleotide phosphodiesterases and cyclic nucleotide-dependent protein kinases. Methods 14:81-92
Turko, I V; Francis, S H; Corbin, J D (1998) Binding of cGMP to both allosteric sites of cGMP-binding cGMP-specific phosphodiesterase (PDE5) is required for its phosphorylation. Biochem J 329 ( Pt 3):505-10
Loughney, K; Hill, T R; Florio, V A et al. (1998) Isolation and characterization of cDNAs encoding PDE5A, a human cGMP-binding, cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase. Gene 216:139-47
Granovsky, A E; Natochin, M; McEntaffer, R L et al. (1998) Probing domain functions of chimeric PDE6alpha'/PDE5 cGMP-phosphodiesterase. J Biol Chem 273:24485-90
Wyatt, T A; Naftilan, A J; Francis, S H et al. (1998) ANF elicits phosphorylation of the cGMP phosphodiesterase in vascular smooth muscle cells. Am J Physiol 274:H448-55

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