Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM041751-07
Application #
2181032
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1989-04-01
Project End
1997-06-30
Budget Start
1995-07-01
Budget End
1997-06-30
Support Year
7
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of California Santa Barbara
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Santa Barbara
State
CA
Country
United States
Zip Code
93106
Dougherty, C A; Himes, R H; Wilson, L et al. (1998) Detection of GTP and Pi in wild-type and mutated yeast microtubules: implications for the role of the GTP/GDP-Pi cap in microtubule dynamics. Biochemistry 37:10861-5
Sage, C R; Davis, A S; Dougherty, C A et al. (1995) beta-Tubulin mutation suppresses microtubule dynamics in vitro and slows mitosis in vivo. Cell Motil Cytoskeleton 30:285-300
Gupta, M L; Toso, R J; Farrell, K W et al. (1995) Commercial [3H]glutamate contains a contaminant that labels tubulin covalently. Anal Biochem 230:350-3
Sage, C R; Dougherty, C A; Davis, A S et al. (1995) Site-directed mutagenesis of putative GTP-binding sites of yeast beta-tubulin: evidence that alpha-, beta-, and gamma-tubulins are atypical GTPases. Biochemistry 34:7409-19
Davis, A; Sage, C R; Dougherty, C A et al. (1994) Microtubule dynamics modulated by guanosine triphosphate hydrolysis activity of beta-tubulin. Science 264:839-42
Toso, R J; Jordan, M A; Farrell, K W et al. (1993) Kinetic stabilization of microtubule dynamic instability in vitro by vinblastine. Biochemistry 32:1285-93
Burns, R G; Farrell, K W; Surridge, C D (1993) Should the tubulins be members of the GTPase superfamily? Ciba Found Symp 176:248-62;discussion 262-7
Davis, A; Sage, C R; Wilson, L et al. (1993) Purification and biochemical characterization of tubulin from the budding yeast Saccharomyces cerevisiae. Biochemistry 32:8823-35
Stewart, R J; Farrell, K W; Wilson, L (1990) Role of GTP hydrolysis in microtubule polymerization: evidence for a coupled hydrolysis mechanism. Biochemistry 29:6489-98