We propose structural studies on most available filamentous bacterial viruses, on their intracellular precursor complexes between DNA binding proteins and progeny DNAs, and on the genomes of some. All of the viruses have Gram-negative bacteria as hosts, several of which are pathogenic either in plants or animals. All of the viruses have the same basic overall morphology and all contain a single-stranded DNA genome packed in a sheath of thousands of identical subunits. Techniques such as ultracentrifugation, electron microscopy, light scattering, CD and fluorescence spectroscopy, X-ray diffraction, and other techniques will be applied to determine fundamental structure parameters. Studies on interactions, between intact virions between various virion components, and between the DNA binding proteins and nucleic acids are also proposed. The amino acid sequences of the major structural proteins are to be established through DNA sequencing and protein sequencing techniques. Theoretical work will be directed toward unified theories of the structures of these similar, but in detail diverse, nucleoproteins having the same function, and toward understanding of their evolutionary relationships.

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National Institute of General Medical Sciences (NIGMS)
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Biophysical Chemistry Study Section (BBCB)
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Public Health Research Institute
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Shin, S; Day, L A (1995) Separation and size determination of circular and linear single-stranded DNAs by alkaline agarose gel electrophoresis. Anal Biochem 226:202-6
Kostrikis, L G; Reisberg, S A; Kim, H Y et al. (1995) C2, and unusual filamentous bacterial virus: protein sequence and conformation, DNA size and conformation, and nucleotide/subunit ratio. Biochemistry 34:4077-87
Kostrikis, L G; Liu, D J; Day, L A (1994) Ultraviolet absorbance and circular dichroism of Pf1 virus: nucleotide/subunit ratio of unity, hyperchromic tyrosines and DNA bases, and high helicity in the subunits. Biochemistry 33:1694-703
Marzec, C J; Day, L A (1994) An electrostatic spatial resonance model for coaxial helical structures with applications to the filamentous bacteriophages. Biophys J 67:2205-22
Liu, D J; Day, L A (1994) Pf1 virus structure: helical coat protein and DNA with paraxial phosphates. Science 265:671-4
Marzec, C J; Day, L A (1993) Pattern formation in icosahedral virus capsids: the papova viruses and Nudaurelia capensis beta virus. Biophys J 65:2559-77
Hendrix, R; Madras, N; Johnson, R (1993) Growth pressure can drive early chick lens geometries. Dev Dyn 196:153-64
Marzec, C J; Day, L A (1993) An exact description of five-membered ring configurations. II. Applications to furanose rings in DNA and RNA, analysis of errors, and bond angle bending energy. J Biomol Struct Dyn 10:1125-54
Marzec, C J; Day, L A (1993) An exact description of five-membered ring configurations. I. Parameterization via an amplitude S, an angle gamma, the pseudorotation amplitude q and phase angle P, and the bond lengths. J Biomol Struct Dyn 10:1091-123
Kostrikis, L G; Reisberg, S A; Simon, M N et al. (1991) Export of infectious particles by Escherichia coli transfected with the RF DNA of Pf1, a virus of Pseudomonas aeruginosa strain K. Mol Microbiol 5:2641-7