Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
7R01GM042645-05
Application #
3301395
Study Section
Molecular and Cellular Biophysics Study Section (BBCA)
Project Start
1989-07-01
Project End
1997-03-31
Budget Start
1994-01-01
Budget End
1994-03-31
Support Year
5
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Palo Alto Institute/Molecular Medicine
Department
Type
DUNS #
City
Mountain View
State
CA
Country
United States
Zip Code
94043
Coleman, W J; Mattioli, T A; Youvan, D C et al. (1997) Site-directed mutations near the L-subunit D-helix of the purple bacterial reaction center: a partial model for the primary donor of photosystem II. Biochemistry 36:2178-87
Yang, T T; Kain, S R; Kitts, P et al. (1996) Dual color microscopic imagery of cells expressing the green fluorescent protein and a red-shifted variant. Gene 173:19-23
Mitra, R D; Silva, C M; Youvan, D C (1996) Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein. Gene 173:13-7
Delagrave, S; Goldman, E R; Youvan, D C (1995) Context dependence of phenotype prediction and diversity in combinatorial mutagenesis. Protein Eng 8:237-42
Delagrave, S; Hawtin, R E; Silva, C M et al. (1995) Red-shifted excitation mutants of the green fluorescent protein. Biotechnology (N Y) 13:151-4
Delagrave, S; Goldman, E R; Youvan, D C (1993) Recursive ensemble mutagenesis. Protein Eng 6:327-31
Delagrave, S; Youvan, D C (1993) Searching sequence space to engineer proteins: exponential ensemble mutagenesis. Biotechnology (N Y) 11:1548-52
Robles, S J; Youvan, D C (1993) Hydropathy and molar volume constraints on combinatorial mutants of the photosynthetic reaction center. J Mol Biol 232:242-52
Nabedryk, E; Robles, S J; Goldman, E et al. (1992) Probing the primary donor environment in the histidineM200-->leucine and histidineL173-->leucine heterodimer mutants of Rhodobacter capsulatus by light-induced Fourier transform infrared difference spectroscopy. Biochemistry 31:10852-8
Arkin, A P; Youvan, D C (1992) An algorithm for protein engineering: simulations of recursive ensemble mutagenesis. Proc Natl Acad Sci U S A 89:7811-5

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