The overall goal of this work is to elucidate the interaction of linker histones with chromatin. Eight different studies are proposed in four groups. Experiments 1 & 2 use a DNA cleavage assay to determine the location and orientation of GH5 and its C- terminal tail bound to nucleosomes. Experiment 3 measures the Kd for nucleosome binding. Experiments 4 & 5 examine the roles of particular residues in nucleosome binding and in cooperative binding to DNA. Experiment 6 addresses sequence-preferences in DNA binding. Experiments 7 & 8 are proposed studies of protein-DNA co-crystals to elucidate the binding of GH5 and its C-terminal tail.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM042796-07
Application #
2022323
Study Section
Special Emphasis Panel (ZRG3-BBCB (01))
Project Start
1989-07-01
Project End
1999-11-30
Budget Start
1996-12-01
Budget End
1997-11-30
Support Year
7
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Utah
Department
Biochemistry
Type
Schools of Medicine
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
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