Generation of Antibody Diversity

Gearhart, Patricia J.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Research Project (R01)
• Project #: 5R01GM042975-12
• Application #: 2181755
• Study Section: Allergy and Immunology Study Section (ALY)
• Project Start: 1989-01-01
• Project End: 1996-12-31
• Budget Start: 1995-01-01
• Budget End: 1996-12-31
• Support Year: 12
• Fiscal Year: 1995

Institution

• Name: Johns Hopkins University
• Department: Biochemistry
• Type: Schools of Public Health
• DUNS #: 045911138
• City: Baltimore
• State: MD
• Country: United States
• Zip Code: 21218

Publications

Winter, D B; Sattar, N; Mai, J J et al. (1997) Insertion of 2 kb of bacteriophage DNA between an immunoglobulin promoter and leader exon stops somatic hypermutation in a kappa transgene. Mol Immunol 34:359-66

Umar, A; Gearhart, P J (1995) Reciprocal homologous recombination in or near antibody VDJ genes. Eur J Immunol 25:2392-400

Lawler, A M; Umar, A; Gearhart, P J (1992) Linkage of two pseudogenes from V kappa 1 and V kappa 9 murine immunoglobulin families. Mol Immunol 29:295-301

Umar, A; Schweitzer, P A; Levy, N S et al. (1991) Mutation in a reporter gene depends on proximity to and transcription of immunoglobulin variable transgenes. Proc Natl Acad Sci U S A 88:4902-6

Lebecque, S G; Gearhart, P J (1990) Boundaries of somatic mutation in rearranged immunoglobulin genes: 5' boundary is near the promoter, and 3' boundary is approximately 1 kb from V(D)J gene. J Exp Med 172:1717-27

Lawler, A M; Kearney, J F; Kuehl, M et al. (1989) Early rearrangements of genes encoding murine immunoglobulin kappa chains, unlike genes encoding heavy chains, use variable gene segments dispersed throughout the locus. Proc Natl Acad Sci U S A 86:6744-7