Current models for the higher order organization of eukaryotic chromosomes have suggested that the DNA-chromatin fiber is organized into a series of looped domains. A critical feature of the model are the nucleoprotein structures at the boundaries of each loop which organize the chromatin fiber into discrete domains. We have identified and characterized a novel class of chromatin structures, called scs, which appear to correspond to boundaries of higher chromosomal domains. We have shown that the scs- structures provide in vivo sites of action for topo II under circumstances where the topology of the domain is altered by gene activation/de- activation. We have also shown that the scs-structures can insulate against position effects, and will function to block the activity of nearby enhancer elements. The primary goals of the experiments proposed here are 1) to identify and characterize proteins which bind to scs DNA segments in vitro, 2) demonstrate that these proteins are associated with scs in vivo, and that this association is important for scs function, 3) characterize the scs nucleoprotein complex in detail, and 4) develop genetic strategies for analyzing how the scs protein complex functions in vivo.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM043432-01
Application #
3302472
Study Section
Molecular Biology Study Section (MBY)
Project Start
1989-12-01
Project End
1994-11-30
Budget Start
1989-12-01
Budget End
1990-11-30
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Princeton University
Department
Type
Schools of Arts and Sciences
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544
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