Abstract

This is a revised proposal of a competing renewal. Five years of research are proposed in which experiments will be carried out dealing with four specific aims: 1. Biochemical studies of RTs encoded by retrons Ec73 and Ec86; 2. Exploration of possible functions of retrons in E. coli cells; 3. Studies of retrons as mobile elements; 4. In vivo synthesis of oligodeoxyribonucleotides using retrons as a vector.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM044012-09
Application #
2684939
Study Section
Special Emphasis Panel (ZRG5-BM-1 (02))
Project Start
1990-04-01
Project End
2000-03-31
Budget Start
1998-04-01
Budget End
1999-03-31
Support Year
9
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Medicine & Dentistry of NJ
Department
Biochemistry
Type
Schools of Medicine
DUNS #
622146454
City
Piscataway
State
NJ
Country
United States
Zip Code
08854

  Publications

Lampson, B C; Inouye, M; Inouye, S (2005) Retrons, msDNA, and the bacterial genome. Cytogenet Genome Res 110:491-9
Lampson, Bert C; Xu, Chunying; Rice, Scott A et al. (2002) A partial copy of msDNA from a new retron element is likely a retrotransposed DNA found in the myxobacterium Nannocystis exedens. Gene 299:251-61
Mirochnitchenko, O; Inouye, M (2000) Antisense RNA and DNA in Escherichia coli. Methods Enzymol 313:467-85
Inouye, S; Hsu, M Y; Xu, A et al. (1999) Highly specific recognition of primer RNA structures for 2'-OH priming reaction by bacterial reverse transcriptases. J Biol Chem 274:31236-44
Shimamoto, T; Kawanishi, H; Tsuchiya, T et al. (1998) In vitro synthesis of multicopy single-stranded DNA, using separate primer and template RNAs, by Escherichia coli reverse transcriptase. J Bacteriol 180:2999-3002
Mao, J R; Inouye, S; Inouye, M (1997) msDNA-Ec48, the smallest multicopy single-stranded DNA from Escherichia coli. J Bacteriol 179:7865-8
Inouye, M; Mao, J R; Shimamoto, T et al. (1997) In vivo production of oligodeoxyribonucleotides of specific sequences: application to antisense DNA. Ciba Found Symp 209:224-33; discussion 233-4
Mao, J R; Inouye, M; Inouye, S (1996) An unusual bacterial reverse transcriptase having LVDD in the YXDD box from Escherichia coli. Biochem Biophys Res Commun 227:489-93
Mao, J R; Inouye, S; Inouye, M (1996) Enhancement of frame-shift mutation by the overproduction of msDNA in Escherichia coli. FEMS Microbiol Lett 144:109-15
Inouye, S; Inouye, M (1995) Structure, function, and evolution of bacterial reverse transcriptase. Virus Genes 11:81-94

Showing the most recent 10 out of 29 publications