Oxygen is a fundamental regulator of cellular bioenergetics and intermediary metabolism. Observing tissue oxygen level, however, has faced many hurdles. Extant techniques confront major obstacles in sampling intracellular oxygenation in a localized tissue region. Consequently a key question cannot be posed: Is the accepted view of the interaction between oxygen level and the regulation of bioenergetics, derived from model studies, also accurate in vivo? NMR offers a unique opportunity to answer such questions in vivo. Our preliminary data have demonstrated the 1H NMR visibility of the myoglobin proximal histidyl F8 NH and Val Ell methyl groups in heart muscle. These signals directly reflect intracellular oxygenation, in contrast to the commonly used 31P peaks, which monitor only the cellular interaction. We propose then to establish firmly the 1H NMR techniques to observe oxygen tension in specific tissue regions in vivo. Clearly the capacity to measure tissue oxygen opens many applications with both physiological and pathophysiological perspectives.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM044916-03
Application #
2182865
Study Section
Biophysical Chemistry Study Section (BBCB)
Project Start
1992-02-01
Project End
1997-01-31
Budget Start
1994-02-01
Budget End
1995-01-31
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of California Davis
Department
Biochemistry
Type
Schools of Medicine
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
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Kreutzer, U; Jue, T (1996) H-NMR signal of Arenicola marina myoglobin in vivo as an index of tissue oxygenation. Eur J Biochem 235:622-8
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Kreutzer, U; Jue, T (1995) Critical intracellular O2 in myocardium as determined by 1H nuclear magnetic resonance signal of myoglobin. Am J Physiol 268:H1675-81
Busse, S C; Jue, T (1994) Two-dimensional NMR characterization of the deoxymyoglobin heme pocket. Biochemistry 33:10934-43
Jue, T; Kreutzer, U; Chung, Y (1994) 1H NMR approach to observe tissue oxygenation with the signals of myoglobin. Adv Exp Med Biol 361:111-8

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