The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe provide excellent model systems for studying cell cycle regulation, because they have fairly typical eukaryotic cell cycles displaying a variety of controls, and because they are easy to analyze by a host of powerful genetic and biochemical techniques. Previous studies have focused on a mitotic control point in S. pombe, and a Gl control point in S. cerevisiae. We believe that each yeast (and eukaryotes in general) actually has a control at both points. Further, we believe the two controls are homologous to each other. We call this the Twin Clock Hypothesis, and our goal is to prove it by finding the components of both clocks in a single system. An additional goal of this project is to study the roles and the regulation of the Cdc2/Cdc28 family of protein kinases.
| Nash, R S; Volpe, T; Futcher, B (2001) Isolation and characterization of WHI3, a size-control gene of Saccharomyces cerevisiae. Genetics 157:1469-80 |
| Futcher, B; Latter, G I; Monardo, P et al. (1999) A sampling of the yeast proteome. Mol Cell Biol 19:7357-68 |
| Amon, A; Tyers, M; Futcher, B et al. (1993) Mechanisms that help the yeast cell cycle clock tick: G2 cyclins transcriptionally activate G2 cyclins and repress G1 cyclins. Cell 74:993-1007 |
| Fitch, I; Dahmann, C; Surana, U et al. (1992) Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae. Mol Biol Cell 3:805-18 |
| Tyers, M; Fitch, I; Tokiwa, G et al. (1991) Characterization of G1 and mitotic cyclins of budding yeast. Cold Spring Harb Symp Quant Biol 56:21-32 |
| Xiong, Y; Connolly, T; Futcher, B et al. (1991) Human D-type cyclin. Cell 65:691-9 |
| Futcher, A B (1991) Saccharomyces cerevisiae cell cycle: cdc28 and the G1 cyclins. Semin Cell Biol 2:205-12 |
